红菇娘和紫菇娘的组织培养与繁殖  

Tissue Culture and Reproduction of Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao

作　　者：王波[1] 李用 吕晓文 姜成[2] 薛春梅[1] 杜春梅[1] 程海涛[2] 邵红[2] 孙长利 韩诚武[1] 

机构地区：[1]佳木斯大学微生物研究所,黑龙江佳木斯154007 [2]佳木斯大学,黑龙江佳木斯154007 [3]佳木斯第二中学,黑龙江佳木斯154002

出　　处：《安徽农业科学》2018年第4期97-99,共3页Journal of Anhui Agricultural Sciences

基　　金：佳木斯大学校级科研课题(S2014-029)

摘　　要：[目的]筛选野生红菇娘(Calyx seu Fructus Physalis)和紫菇娘(Physalis tungkenensis Kuan et Gao)组织培养的最佳培养基。[方法]分别以2种植物的茎、叶为外植体,比较不同取样方式、不同增殖条件及不同生根条件对2种植物组织培养和快速繁殖的影响。[结果]以MS+6-BA 3.0 mg/L+NAA 0.04 mg/L为最适合红菇娘愈伤组织诱导和分化的培养基,而紫菇娘是MS+6-BA 3.0 mg/L和NAA0.03 mg/L,培养14 d后每个愈伤组织能产生3~5个不定芽。继代和生根培养后,丛生芽的成活率为90%以上。[结论]该研究可为2种酸浆属野生种质资源保存和优质种苗生产提供技术参考。[Objective]The best tissue culture medium for Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao was selected.[Method]Stem and leaf were used as the explants for the tissue culture and rapid propagation by comparing different sampling methods,different breeding conditions and different rooting conditions.[Result]MS + 6-BA 3. 0 mg/L + NAA 0. 04 mg/L was the best medium for callus induction and bud differentiation of Calyx seu Fructus Physalis,MS + 6-BA3. 0 mg/L + NAA 0. 03 mg/L was the best medium for callus induction and bud differentiation of Physalis tungkenensis Kuan et Gao,there were 3 and 5 clustered shoots per one callus induction for both Calyx seu Fructus Physalis and Physalis tungkenensis Kuan et Gao respectively after 14 days. The survival rate reached 90% after subculturing and rooting culture.[Conclusion]The study can provide technical reference for the preservation of wild germplasm resources and the production of high-quality seedlings.

关 键 词：红菇娘 紫菇娘 组织培养 愈伤组织 

分 类 号：S641.4[农业科学—蔬菜学]