预先应用甲状腺素片对脓毒症大鼠肿瘤坏子因子-α和肺组织病理的影响  

Effect of thyroxine pretreatment on tumor necrosis factor -α and lung pathology in septic rats

作　　者：邹文茂 李景辉[1] 刘蕾 刘芙蓉[1] 邓超[1] 敖雪[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院重症医学科,海南海口570208

出　　处：《中国急救医学》2018年第2期179-183,I0002,共6页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨预先应用甲状腺素片对脓毒症大鼠肿瘤坏死因子-α（TNF-α）和肺组织病理的影响。方法大鼠尾静脉注射脂多糖（LPS，5mg／kg）建立脓毒症模型。36只雄性Wistar大鼠随机分为空白对照组（C组）、脓毒症组（S组）、甲状腺素预先干预组（L组），每组12只。L组预先左旋甲状腺素钠（10μg／100g）灌胃7d，S组和c组等量生理盐水灌胃7d。建模后0、3、6、12、24h采血，测血清TNF-a、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）水平，24h处死大鼠并取肺组织，测肺组织湿／干质量比（W／D），行HE染色并免疫组化。结果FT3、FT4在0h各组均无变化（P〉0．05），s组、L组3h降低，6h最低，然后逐渐上升，在3、6、12、24h等时间点较c组明显降低（P〈0．01），而在3、6、12、24h时间点L组较S组明显升高（P〈0．01）。S组和L组W／D较c组升高（P〈0．01），较S组升高更明显（P〈0．01）。病理显示，c组肺泡结构完整；S组肺泡壁塌陷，间隔增宽，炎细胞浸润明显；L组较s组减轻。血清TNF-α在S组和L组3h升高，6h达峰值，较C组升高（P〈0．01），较S组升高更明显（P〈0．01）；肺组织TNF-α阳性细胞数量和染色强度s组最多、最强，L组次之，c组最少、最弱。结论预先应用甲状腺素片能降低脓毒症大鼠血清TNF-α水平，改善肺组织病理改变。Objective To investigate the effect of thyroxine pretreatment on tumor necrosis factor-ct （TNF- a） and lung pathology in septic rats. Methods LPS （5 mg/kg） was injected into the caudal vein of rats to establish the sepsis model. Thirty - six male Wistar rats were randomly divided into blank control group （group C ） , sepsis group （group S ） and thyroxine pretreatment group （group L） , with 12 rats in each group. Group L was given by gavage with levothyroxine sodium （ 10 μg/100 g） for 7 days, and equivalent physiological saline were administered in the same protocol without levothyroxine sodium for 7 days in group S and group C. Rats in each group were drew blood after modeling for 0, 3, 6, 12, 24 h. And the levels of TNF - a, free three triiodothyro （ FT3 ） and free thyroxine （FT4） were measured. Rats were sacrificed after 24 h and lung tissue was taken to measure the wet/dry weight （W/D） , and HE staining and immunohistochemistry were performed. Results The level of FT3 and FT4 was not significanlg different among each group at 0 h （ P 〉 0.01 ）. However itbegan to decrease at 3 h, and decreased to a minimum at 6 h in group S and group L. The FT3, FT4 level in group L and group S was lower than that in group C （ P 〈 0.01 ）, and reduced more markedly in group S （P〈0.01）. The W/D in group S and group L was higher than that in group C （P〈0.01）, and elevated more markedly in group S （P 〈 0.01 ）. The pathological findings showed that the alveolar structure of group C was intact. However in group S, the alveolar wall collapsed with alveolar septum broadening, and the inflammatory cell infiltration was obvious. Compared with group S, the pathological changes of group L were obviously reduced. The serum TNF -α concentration started to elevate at 3 h, and reached a peak at 6 h in group S and group L. It was higher than that in group C （P 〈 0.01 ） , and elevated more markedly in group S （ P 〈 0.01 ）. The TNF -α positive cell number and stainin

关 键 词：甲状腺素 脓毒症 非甲状腺疾病综合征(NTIS) 肺 肿瘤坏死因子-α(TNF-α) 

分 类 号：R255.1[医药卫生—中医内科学]