BAY11-7082对严重烧伤大鼠急性肺损伤及早期肺炎症反应的干预作用  

lnterventional effects of BAYll-7082 on lung inflammatory response at the early stage and acute lung injury of rats with severe burns

作　　者：李虎山[1] 杨薛康 郝振明[1] 雷晋[1] 

机构地区：[1]山西医科大学第六医院烧伤科、山西省烧伤救治中心,太原030009 [2]空军军医大学西京医院全军烧伤中心、烧伤与皮肤外科

出　　处：《中华烧伤杂志》2018年第2期88-95,共8页Chinese Journal of Burns

摘　　要：目的探讨BAY11－7082对严重烧伤大鼠急性肺损伤及早期肺炎症反应的干预作用。方法（1）实验1。取12只SD大鼠，按随机数字表法分为正常对照组3只、烧伤组9只。正常对照组大鼠不行特殊处理；烧伤组大鼠背部造成30%体表总面积Ⅲ度烧伤，伤后即刻腹腔注射生理盐水50 mL/kg。烧伤组于伤后12、24、48 h分别取3只大鼠采集腹主动脉血并取肺组织，酶联免疫吸附测定法检测血清中白细胞介素1β（IL－1β）、IL－18含量，实时荧光定量反转录－聚合酶链反应（RT－PCR）法检测肺组织中IL－1β、IL－18 mRNA表达。正常对照组大鼠同前采样检测。（2）实验2。取18只SD大鼠，按随机数字表法分为正常对照组、单纯烧伤组、BAY11－7082干预组，每组6只。正常对照组大鼠不行特殊处理；单纯烧伤组和BAY11－7082干预组大鼠同实验1致伤，伤后即刻分别腹腔注射生理盐水、终质量浓度为8 mg/mL BAY11－7082溶液50 mL/kg。于实验1结果得出的最佳观察时间点收集烧伤各组大鼠肺组织及肺泡灌洗液，苏木素－伊红染色观察肺组织形态并进行肺组织病理损害评分，酶标仪检测肺组织中髓过氧化物酶（MPO）含量及肺泡灌洗液中总蛋白含量，蛋白质印迹法检测肺组织中半胱氨酸天冬氨酸蛋白酶1和核苷酸结合寡聚化结构域样受体3（NLRP3）的蛋白表达，实时荧光定量RT－PCR法检测肺组织中IL－1β、IL－18、NLRP3、半胱氨酸天冬氨酸蛋白酶1 mRNA表达。正常对照组大鼠同前采样检测。对数据行单因素方差分析、LSD－t检验。结果（1）烧伤组大鼠伤后12、24、48 h血清中IL－1β和IL－18含量显著高于正常对照组（t＝10.55、22.05、12.47、10.60、15.22、11.94，P〈0.01）。烧伤组大鼠伤后12、24、48 h肺组织中IL－1β和IL－18 mRNA表达量明显高于正常对照组（t＝3.62、7.19、5.28、3.20、12.62、7.31，P〈0.05或P〈0.01）。伤�Objective To investigate the interventional effects of BAY11-7082 on lung inflammatory response at the early stage and acute lung injury of rats with severe burns. Methods （1） Experiment 1. Twelve Sprague-Dawley （SD） rats were divided into control （C） group and burn （B） group according to the random number table, with 3 rats in group C and 9 rats in group B. Rats in group C did not receive any special treatment. Rats in group B were inflicted with 30% total body surface area full-thickness burn on the back. Immediately after injury, rats in group B were intraperitoneally injected with normal saline in the dosage of 50 mL/kg. Abdominal aorta blood and lung tissue samples were collected from three rats in group B at post injury hour （PIH） 12, 24, and 48, respectively. The interleukin-1β （IL-1β） and the IL-18 content of serum were determined with enzyme-linked immunosorbent assay. The mRNA expressions of IL-1β and IL-18 in lung tissue were determined with real-time fluorescent quantitative reverse transcription polymerase chain reaction （RT-PCR）. Sample collection and determination in rats of group C were performed as above. （2） Experiment 2. Eighteen SD rats were divided into control （C） group, simple burn （SB） group, and BAY11-7082 intervention （BI） group according to the random number table, with 6 rats in each group. Rats in group C did not receive any special treatment. Rats in groups SB and BI were inflicted with injury as in experiment 1. Immediately after injury, rats in group SB were intraperitoneally injected with normal saline in the dosage of 50 mL/kg, and those in group BI with 8 mg/mL （final mass concentration） BAY11-7082 solution in the dosage of 50 mL/kg. Lung tissue and bronchoalveolar lavage fluid （BALF） of rats with burns were collected at the optimal observation time point concluded from experiment 1. The morphology of lung tissue was observed with hematoxylin-eosin staining, and the pathological damage of lung tissue was graded. The myelo

关 键 词：烧伤 急性肺损伤 炎症 半胱氨酸天冬氨酸蛋白酶1 白细胞介素1Β 白 细胞介素18 核苷酸结合寡聚化结构域样受体3 

分 类 号：R644[医药卫生—外科学]