GC-MS法在老年营养不良患者血浆小分子代谢物质测定中的应用  

Application of GC-MS technique to determination of malnutrition in elderly patients plasma small molecule metabolites qualitative research

作　　者：杨维佳[1] 杨敏春[1] 姚惠[1] 王丽君[1] 郑培奋[1] YANG Wei-jia;YANG Min-chun;YAO Hui;et al(Department of Internal Medicine, Zhefiang Hospital of Traditional Chinese Medicine, Hangzhou , Zhejiang 310013, China)

机构地区：[1]浙江医院中医内科,浙江杭州310013

出　　处：《中华全科医学》2018年第1期135-138,共4页Chinese Journal of General Practice

基　　金：2014年浙江省中医药优秀青年人才基金项目(2014-ZQ001);2014年浙江省医药卫生一般研究计划(A类)(2014KYA004);浙江医院重点学科

摘　　要：目的采用GC-MS法测定老年营养不良患者血浆小分子代谢物质,寻找与老年患者营养不良发生发展密切相关的小分子代谢物,探讨代谢组学在老年营养不良患者中的应用。方法选取2015年2月—2016年2月在浙江医院诊治的30例老年营养不良患者作为实验组,同期按年龄、性别匹配选取30例营养状况正常的老年人作为对照组,分别采集2组研究对象的晨起空腹静脉血5 ml,静置分离血浆,分装低温保存。采用GC-MS法测定2组研究对象血浆小分子代谢物质,采用主成分分析和最小方差判别分析法对2组研究对象血浆小分子代谢物质进行统计分析,并比较2组血浆小分子代谢物质水平。结果 2组研究对象血浆总离子流色谱图基本相似,色谱保留时间主要集中在5~15 min,实验组峰高明显低于对照组。实验组与对照组样品主成分得分图可以明显区分。实验组和对照组的置换检验截距R2=0.668,Q^2=-0.309。实验组甘氨酸、天冬氨酸、苏糖酸的值较对照组升高,而鸟氨酸、油酸、尿酸、D-塔罗糖、N-甲基-DL-丙氨酸、甘露糖、D-甘油磷酸酯、β-丙氨酸、α-酮戊二的值较对照组低(P<0.05)。结论 GC-MS法能检测出与老年营养不良患者有关的小分子代谢物质种类,提示GC-MS法可作为老年营养不良患者小分子代谢物检测的筛选方法。Objective To determinate small molecules in elderly patients with malnutrition plasma metabolic substances by using GC-MS method, looking for is closely related to the malnutrition in elderly patients with development of small molecule metabolites ; to discuss application of metabonomics in elderly patients with malnutrition. Methods From Febmary, 2015 to February, 2016 in our hospital diagnosis and treatment of 30 cases of malnutrition in elderly patients were selected as experimental group, during the same period by age, gender, match the old people in 30 cases of normal nutri- tional status as the control group. Acquisition of both groups respectively since morning fasting venous blood 5 ml, let stand separation blood plasma, repackaging kept at low temperature. The two groups was evaluated by GC-MS method plasma small molecule metabolic substances. Using principal component analysis and diseriminant analysis on two groups of minimum variance research object plasma statistical analysis of small molecules metabolic substances small molecules and compare the two groups of plasma metabolic material level. Results Both groups blood plasma total ion current ehromatogram basic similar. The chromatographic retention time are mainly concentrated in 5 - 15 min, The experimental group was significantly lower than the control group. The experimental way and the control sample principal component score chart can clearly distinguish. The experimental group and control group permutation test intercept R2 = 0. 668, Q2 = 0.309. glycine, aspartic acid. Sue sugar acid response values of the experimental group were higher than the control group, while ornithine, oleic acid, uric acid, D-talose, N-methyl-DL-alanine, mannose, D-glyeerophosphate, alpha and beta alanine glutaric response values to lower（P 〈 0.05 ）. Condusion GC-MS method can detect small molecules related to the elderly patients with malnutrition metabolic material types, Prompt GC-MS method can be used as in elderly patients with malnutrition screening of sma

关 键 词：GC-MS法 老年患者 营养不良 小分子代谢物 

分 类 号：R161.7[医药卫生—公共卫生与预防医学] R153.3