百合转P5CS-F129A基因的遗传稳定性和耐旱性检测  

Inheritance stability and drought stress test for P5CS-F129A transgenic lily

作　　者：闫笑 魏迟 张冬梅 贾桂霞[1] Yan Xiao;Wei Chi;Zhang Dong-mei;Jia Gui-xia(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation ＆ Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,School of Landscape Architecture,Beijing Forestry University,COFCO（Beijing） Agri-Ecological Valley Development Co.,Ltd,Shanghai Academy of Landscape Architecture Science and Planning,Shanghai Engineering Research Center of Landscaping on Challenging Urban Site)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,林木花卉遗传育种教育部重点实验室,北京林业大学园林学院,北京100083 [2]北京市中粮北京农业生态谷发展有限公司,北京102403 [3]上海市园林科学规划研究院,上海城市困难立地绿化工程技术研究中心,上海200231

出　　处：《北京林业大学学报》2018年第2期98-105,共8页Journal of Beijing Forestry University

基　　金：“863”国家高技术研究发展计划项目(2013AA102706)

摘　　要：【目的】百合是世界上主要的商品花卉之一,具有很高的观赏价值和经济价值,然而目前的主栽东方百合品种耐旱和耐盐碱性较差,对其进行改良是提高百合价值的关键因素。【方法】对已获得的含抗逆相关基因P5CSF129A的东方百合‘索邦’单拷贝转化株系L89和双拷贝株系L31进行了4年的组培继代培养和1年的温室栽培,检测了目的基因的稳定性和干旱胁迫处理下的表型和生理指标的变化。经Southern印迹杂交和c DNA检测显示目的基因能够稳定遗传。【结果】干旱胁迫处理结果表明:两个转基因株系叶片脯氨酸含量均高于普通‘索邦’,其中株系L31的脯氨酸水平最高;虽然干旱胁迫下两个转基因株系和普通‘索邦’叶片相对电导率均有所升高,但转基因株系叶片的相对电导率低于普通‘索邦’,重新灌水后,转基因株系恢复正常水平,普通‘索邦’未恢复;在表型性状等方面,经过胁迫并复水的株系L89和L31总体长势良好,两株系之间长势无显著差异,而普通‘索邦’叶片黄化严重,生长受阻。【结论】转P5CS-F129A基因植株具有更强的耐旱性和遗传稳定性,对抗逆性百合新品种的培育具有重要意义。[Objective]Lilium spp.is one of the main commercial flowers in the world which possesses both high ornamental and economic value.However,the current leading cultivars of Lilium Oriental hybrid are lack of drought and saline-alkaline tolerance thus it is crucial to improve the above properties.[Method]Based on four-year subculture and one-year greenhouse culture,the stability of the target gene and changes of the phenotypes and physiological indices were tested for the single copytransformation line L89 and double copy line L31 with the P5 CS-F129 A gene of Lilium Oriental hybrid＇Sorbonne＇.The Southern blot and c DNA detection showed stable inheritance of the target gene.[Result]The results of drought stress treatments demonstrated that the leaves of both transgenic lines contained higher proline content than the common ＇Sorbonne＇,of which L31 contained the highest proline level.Though the relative leaf ion leakage of both transgenic lines and the common ＇Sorbonne＇rose in the drought stress process,the transgenic lines showed lower relative leaf ion leakage than the common ＇Sorbonne＇.After the second watering,the relative leaf ion leakage of both transgenic lines recovered to normal level,while the common ＇Sorbonne＇didn＇t.In the aspect of phenotype,both L89 and L31 grew well after the second watering,showing no obvious damage on growth,while the common＇Sorbonne＇showed severe leaf etiolation and growth retardation.[Conclusion]The above research indicated that the P5 CS-F129A transgenic gene lines possessed stronger drought tolerance which would be significant to the cultivation of new cultivars of stress-tolerant lily.

关 键 词：东方百合 P5CS-F129A 转基因 干旱胁迫 

分 类 号：S682.2[农业科学—观赏园艺]