灯盏花素对顺铂肾损害小鼠肾小球作用的观察  

Observation on the effect of breviscapine on glomeruli in mice with cisplatin-induced renal damage

作　　者：任翔[1] 任亮[2] 刘金宝[2] 朱吾元[2] 

机构地区：[1]中国药科大学,南京211198 [2]漯河医学高等专科学校药学系,462000

出　　处：《国际医药卫生导报》2018年第2期149-155,共7页International Medicine and Health Guidance News

基　　金：河南省科技攻关计划项目（132102310455）

摘　　要：目的观察灯盏花素对顺铂肾损害小鼠模型肾小球及肾小球基底膜的保护作用。方法将C57BL／6J小鼠随机分为空白组、模型组、25mg及50mg灯盏花素实验组。除空白组外，其余各组腹腔注射顺铂8mg·kg^-1制备小鼠顺铂肾损害模型。两个实验组分别连续灌胃25mg·kg^-1及50mg·kg^-1的灯盏花素10d后处死小鼠，HE法及PAS法观察小鼠。肾小球的病理变化并进行评分，Weternbloting法检测肾组织Ⅳ型胶原蛋白（Col-IV）、纤维连接蛋白（FN）及层粘连蛋白（LN）的表达。结果与空白组比较，模型组出现肾小球细胞数增加、肾小球体积增大、肾小球硬化、球囊壁粘连等，肾小球病理指标存在显著差异（P〈0．05，P〈0．01），肾小球基底膜Col1V、FN、LN表达明显缺失（P〈0．01）；低剂量及高剂量灯盏花素实验组的上述肾损害现象减轻，肾小球病理指标的积分显著低于模型组，。肾小球基底膜Col-IV、FN、LN表达较模型组显著性增强（P〈0．05，P〈0．01）。结论灯盏花素对于顺铂造成的小鼠肾小球损伤具有较好保护作用，且对肾小球基底膜损伤具有一定修复作用。Objective To observe the protective effect ofbreviscapine on the glomerulus and glomerular basement membrane in mice with eisplatin-induced renal damage. Methods Thirty-two C57BL/6J mice were randomly divided into a blank control group, a model group, a 25 mg breviscapine experimental group, and a 50 mg breviscapine experimental group. Except for the blank control group, the other 3 groups were intraperitoneally injected cisplatin 8 mgokg-~ to prepare cisplatin renal damage model in mice. For the 2 experimental groups, gavage was performed in 25 mgokg-~ and 50 mg^kg-~ breviscapine for 10 d, then the mice were sacrificed. HE and PAS staining methods were used to observe the pathological changes of glomernlar and make a score. Western Bloting method was used to detect the expressions of collagen type IV （Col- IV ）, fibronectin （FN）, and laminin （LN）. Results Compared with the blank control group, the model group showed increased number of glomerular cells, volume, and glomernlar sclerosis, adhesion of Bowman＇s capsule （P 〈 0.05, P 〈 0.01）. The expressions of Col-IV, FN, and LN had obvious gene deletion （P 〈 0.01）. In the low dose and high dose of breviscapine in the experimental groups, the renal damage was relieved and the integral glomerular pathological indicators were significantly lower than those in the model group. The expressions of Col-IV, FN, and LN increased significantly in the glomerular basement membrane compared the experimental groups with the model group （P 〈 0.05, P 〈 0.01）. Conclusion Breviscapine has a good protective effect on cisplatin-induced glomerular damage in mice and a certain repair effect on glomerular basement membrane damage.

关 键 词：灯盏花素 顺铂 肾损害 肾小球 基底膜 

分 类 号：R285.5[医药卫生—中药学]