多壁碳纳米管修饰玻碳电极上绿原酸催化氧化谷胱甘肽  

Electrocatalytic Oxidation of Glutathione by Chlorogenic Acid at Au-MWCNTs Modified Glassy Carbon Electrode

作　　者：黄颖[1] 陈美 

机构地区：[1]福建师范大学化学与材料学院,福建福州350007

出　　处：《分析科学学报》2018年第1期27-31,共5页Journal of Analytical Science

摘　　要：制备了纳米Au-多壁碳纳米管复合材料修饰玻碳电极(Au-MWCNTs/GCE),研究了还原型谷胱甘肽(GSH)在该修饰电极上以绿原酸(CGA)为介质的电催化氧化行为。用透射电镜对MWCNTs和Au-MWCNTs进行了表征,结果显示Au纳米粒子均匀地负载在MWCNTs上。电化学测试表明,在磷酸盐缓冲溶液(pH=6.0)中CGA对GSH具有良好的催化作用,用差分脉冲伏安法(DPV)测得GSH氧化峰电流与其浓度在0.1~5.0μmol/L和5.0~30.0μmol/L范围内呈良好线性关系,检测限(S/N=3)为0.034μmol/L。将该方法用于滴眼液中GSH含量的测定,回收率范围为98.0%~102.0%。A simple and novel electrochemical method was proposed ior electrocatalytlc oxadation ot glutatnlone （GSH） using chlorogenic acid（CGA） as a mediator at gold nanoparticles and multi-walled carbon nanotubes composite modified glass carbon electrode（Au-MWCNTs/GCE）. The morphology and structure of MWCNTs and Au-MWCNTs were characterized by transmission electron microscopy（TEM）. The results indicated that gold nanoparticle highly dispersed on MWCNTs. The electrochemical methods demonstrated that CGA showed efficient catalytic activity towards GSH at Au-MWCNTs/GCE. Under the optimal conditions, the oxidation peak current of GSH was linear in its concentration ranges of 0.1-5.0 μmol/L and 5.0- 30.0 μmol/L with a detection limit（S/N= 3） of 0. 034 μmol/L by differential pulse voltammetry（DPV）. The established method was successfully applied to the determination of GSH in reduced glutathione eye drops with the recoveries of 98.0%-102.0%.

关 键 词：谷胱甘肽 绿原酸 金纳米粒子 多壁碳纳米管 

分 类 号：O657.1[理学—分析化学]