黄芩苷在Nb_2O_5-石墨烯纳米复合材料修饰电极上的电化学行为及测定  

Electrochemical Behaviors and Determination of Baicalin at a Nb_2O_5-RGO Nanocomposites Modified Electrode

作　　者：瞿万云 吴艳 胡卫兵 

机构地区：[1]湖北民族学院化学与环境工程学院,湖北恩施445000 [2]湖北民族学院生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《分析科学学报》2018年第1期90-94,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21465009;21461008)

摘　　要：本文采用一步溶剂热法制备Nb2O5-石墨烯(Nb2O5-RGO)纳米复合材料,用于构建一种新型的黄芩苷电化学传感器。用扫描电子显微镜(SEM)对Nb2O5-RGO复合材料进行形貌表征。采用循环伏安法(CV)和线性扫描伏安法(LSV)研究了黄芩苷在Nb2O5-RGO修饰电极上的电化学行为。结果发现,Nb2O5-RGO修饰电极对黄芩苷具有显著的电催化活性。用差分脉冲伏安法(DPV)对黄芩苷进行测定,其氧化峰电流与浓度在1.0×10-5~1.5×10-6 mol·L-1和1.0×10-6~5.0×10-8 mol·L-1范围内呈良好的线性关系,检出限(S/N=3)为1.5×10-8 mol·L-1。该修饰电极的选择性高、重复性和稳定性较好。用于测定黄芩颗粒中黄芩苷的含量,加标回收率为93.0%~95.1%。A Nb2O5-reduced graphene oxide（RGO） modified electrode for electroanalysis of baicalin was fabricated. The composite material was synthesized by a hydrothermal reduction using NbCls and graphene oxide（GO）, and its morphology was characterized by scanning electron microscopy（SEM）. The electrochemical behavior of baicalin was investigated in Britton-Robinson（B-R） buffer solution （pH= 2.1） by cyclic voltammetry（CV） and linear sweep voltammetry（LSV） techniques. The results showed that the Nbe O5-RGO modified electrode exhibited an electrocatalytic activity to baicalin. The oxidation peak current of baicalin had good linear relationship with concentration at 1.0 X 10-5 - 1.5 X 10-6 mol· L-1 and 1.0X10 6-5. 0X10 8 mol·L-1,and the detection limit（S/N=3） was 1. 5 X 10-8 mol. L-1 by differential pulse voltammetry （DPV）. Moreover, this proposed sensor also displayed excellent selectivity,good stability and reproducibility. The proposed method was applied to the determination of baicalin in pharmaceutical products,and the recoveries were in the range of 93.0%-95.1%.

关 键 词：纳米复合材料 石墨烯 黄芩苷 Nb2O5纳米棒 

分 类 号：O657.1[理学—分析化学]