与急性心肌缺血损伤相关体表敏化穴位的初步分布观察  

Preliminary observation on the distribution of the activated acupoints after acute myocardial ischemia

作　　者：董亚琴[1] 黄倩茹[1] 万隆[1] 陈铭[1] 康佩芝 许金森[1] DONG Ya-qin;HUANG Qian-ru;WAN Long;CHEN Ming;KANG Pei-zhi;XU Jin-sen(Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China;College of Acupuncture and Moxibustion, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China)

机构地区：[1]福建省中医药研究院,福州350003 [2]福建中医药大学针灸学院,福州350108

出　　处：《中华中医药杂志》2018年第2期672-674,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81403490;No.81573886);福建省科技厅省属公益类项目(No.2015R1035-3);福建省卫生厅课题(No.2014-ZQN-JC-33);福建省教育厅中青年教师教育科研B类项目(No.JB14050);福建省经络感传重点实验室资助~~

摘　　要：目的:观察与急性心肌缺血损伤相关的体表伊文思蓝(EB)渗出点的分布,并和大鼠穴位的分布进行比较,为疾病状态下的穴位敏化提供实验依据。方法:SD大鼠80只,分为生理盐水对照组(20只)、模型组(60只)。采用背部皮下多部位注射异丙肾上腺素(60mg/kg)造成急性心肌缺血损伤模型(连续2次,间隔24h),当天尾静脉注射EB。观察造模当天(注射完成4h后)EB渗出点的分布。结果:急性心肌缺血损伤造成体表一些部位的神经源性炎性反应点,即EB渗出点,这些反应点的分布与穴位分布呈现一定的相关性;分布位置主要位于:脾、胃俞(74.41%),心俞(60.46%),肝俞(46.51%),肾俞(39.53%)。但这些EB渗出点在正常状态时很少或者不出现。结论:急性心肌缺血损伤可促使EB在体表渗出,渗出点主要分布在脾、胃俞,心俞等穴位,提示在疾病状态下沉寂的穴位可以被激活。Objective：To observe the distribution of the extravasated Evans Blue（EB） dye points（neurogenic inflammatory response） at the skin after acute myocardial ischemia（AMI） and its relation to the related regular acupoints in the locations in rats,to analyze the correlation and to provide experimental basis of activated acupoints during diseases.Methods：A total of 80 SD rats were randomized divided into normal saline group（n=20） and AMI group（n=60）.The AMI group was duplicated by multipoint subcutaneous injection of Isoprinosine（ISO）（60 mg/kg,40 mg/L in 0.9% saline） on the back twice with 24 h interval.Evans Blue dye（50 mg/kg,50 mg/m L in 0.9% saline） was injected into tail vein after the AMI modeling.The plasma extravasated EB points at the skin of the whole body were observed 4 h after injection.Results：Acute myocardial ischemic injury caused neurogenic inflammatory response in some parts of the body surface,which was EB points.The distribution of extravasated EB points showed a certain correlation with the distribution of acupoints.The distribution was mainly located in 74.41% for Pishu/Weishu（BL20/BL21）,60.46% for Xinshu（BL15）,46.51% for Ganshu（BL18）,39.53% for Shenshu（BL3）,respectively.The plasma extravasation of EB seldom appeared in normal rats.Conclusion：AMI promotes the plasma extravasation of EB and the extravasated EB points have a higher corresponding rate with some acupoints including Pishu/Weishu（BL0/BL1）,Xinsu（BL15）,etc.,suggesting an activation of the normally silent acupoints under diseased conditions.

关 键 词：急性心肌缺血损伤 伊文思蓝渗出 敏化穴位 神经源性炎性反应 

分 类 号：R245[医药卫生—针灸推拿学]