和痹方对类风湿关节炎大鼠α7nAChR、STAT3蛋白表达及TNF-α、IL-6、IL-17表达的影响  

Effects of Hebi Recipe on the expression of α7nAChR,STAT3 protein and the expression of TNF-α,IL-6,IL-17 on rheumatoid arthritis rats

作　　者：邢洁[1] 姜萍[1] 姜月华[2] 庞爱梅[2] 牟亮亮 梁占强[1] 张泽 秦林[1] XING Jie;JIANG Ping;JIANG Yue-hua;PANG Ai-mei;MU Liang-liang;LIANG Zhan-qiang;ZHANG Ze;QIN Lin(Shandong University of Chinese Medicine, Jinan 250014, China;Affiliated Hospital of Shandong University of Chinese Medicine, Jinan 250011, China)

机构地区：[1]山东中医药大学,济南250014 [2]山东中医药大学附属医院,济南250011

出　　处：《中华中医药杂志》2018年第2期730-733,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省科技发展计划项目(No.2014GSF119017)~~

摘　　要：目的:探讨和痹方(HBR)通过胆碱能抗炎通路(CAP)治疗类风湿关节炎(RA)的机制。方法:将60只雄性大鼠随机平均分为空白组、模型组、激动剂组、HBR组、甲氨蝶呤(MTX)组、HBR+MTX组,激动剂组药物干预4周,其余组药物干预60d后,取血与关节滑膜,分别检测关节肿胀、肿瘤坏死因子α(TNF-α)、白介素(IL)-6、IL-17、α7烟碱型乙酰胆碱受体(α7nAChR)、转录激活因子3(STAT3)水平。结果:HBR组关节肿胀体积下降,α7nAChR、STAT3水平升高(P<0.05),TNF-α、IL-6、IL-17水平降低(P<0.01)。结论:HBR能够通过CAP,激活JAK2/STAT3信号通路,降低TNF-α、IL-6、IL-17水平,抑制炎性反应,减轻RA的关节肿胀。Objective：To investigate the mechanism of Hebi Recipe（HBR） by cholinergic anti inflammatory pathway（CAP） in the treatment of rheumatoid arthritis（RA）.Methods：Sixty male rats were randomly divided into control group,model group,agonist group and HBR group,methotrexate（MTX） group,HBR＋MTX group.The drug intervention in the agonist group was for 4 weeks,and the other groups were intervened for 60 days.Blood and joint synovial were taken to detect joint swelling,tumor necrosis factor-alpha（TNF-α）,interleukin-6（IL-6）,IL-17,alpha7 nicotinic acetylcholine receptor（α7 n ACh R） and activator of transcription3（STAT3） level.Results：The swelling volume of joint in HBR group decreased,the level of alpha 7nAChR and STAT3 increased（P〈0.05）,and the level of TNF-α,IL-6 and IL-17 decreased（P〈0.01）.Conclusion：HBR could activate JAK2/STAT3 signaling pathway through CAP,reduce the level of TNF-α,IL-6,IL-17,inhibit the inflammatory response,and reduce the swelling of the joint in RA.

关 键 词：类风湿关节炎 胆碱能抗炎通路 和痹方 

分 类 号：R285.5[医药卫生—中药学]