HLA-G基因修饰的胎盘间充质干细胞对CD4^+T淋巴细胞增殖的影响  

Effect of placenta-derived mesenchymal stem cells modified by HLA-G on proliferation of CD4^+T lymphocytes

作　　者：崔桂玉[1] 白剑[2] 苗兰英[2] 林大勇[2] 

机构地区：[1]内蒙古民族大学医学院,内蒙古通辽028000 [2]辽宁中医药大学

出　　处：《山东医药》2018年第4期5-7,共3页Shandong Medical Journal

基　　金：国家自然科学基金面上项目(81571555);辽宁省自然科学基金资助项目(2015020714)

摘　　要：目的探讨人类白细胞抗原G(HLA-G)基因修饰的胎盘间充质干细胞对CD4^+T淋巴细胞增殖的影响。方法自新生儿胚胎分离培养胎盘间充质干细胞并鉴定,将对数生长期的胎盘间充质干细胞随机分为PEGFP-N1-HLA-G组、PEGFP-N1组、对照组,前两组分别转染PEGFP-N1-HLA-G质粒及空载体PEGFP-N1,对照组常规培养,培养20 h时检测HLA-G蛋白相对表达量,结果提示PEGFP-N1-HLA-G质粒促进HLA-G蛋白表达,转染成功。体外分离健康人外周血CD4^+T淋巴细胞,并分别与各组胎盘间充质干细胞共培养。分别于培养24、48和72 h时采用MTT法检测CD4^+T淋巴细胞增殖抑制率。结果 PEGFP-N1-HLA-G组细胞与外周血CD4^+T淋巴细胞共培养24、48、72 h时,CD4^+T淋巴细胞的增殖抑制率均高于对照组和PEGFP-N1组(P均<0.05)。结论 HLA-G基因修饰的胎盘间充质干细胞可抑制CD4^+T淋巴细胞增殖。Objective To investigate the effect＇ of placenta-derived mesenchymal stem cells modified by human leukocyte antigen G （HLA-G） on the proliferation of CD4＋ T lymphocytes. Methods Placenta-derived mesenchymat stem cells from newborn were cultured and identified. Placenta mesenchymal stem cells in logarithmic phase were randomly divided into the PEGFP-N1-HLA-G group, PEGFP-N1 group, and the control group. The former two groups were separately trans- fected with PEGFP-N1-HLA-G plasmid and empty vector PEGFP-N1. The control group was cultured conventionally. After culturing for 20 h, HLA-G protein was detected. The results suggested that PEGFP-N1-HLA-G plasmid promoted the ex- pression of HLA-G protein, and the transfection was successful, In vitro, CD4＋T lymphocytes were isolated from healthy people peripheral blood and mixed with the cells in each group. After culturing for 24, 48 and 72 h, the inhibition rate of CD4＋ T lymphocyte growth was detected by MTT assay, respectively. Results The proliferation inhibition rate of CD4＋ T lymphocytes in the PEGFP-N1-HLA-G group was higher than that in the control group and PEGFP-N1 group at 24, 48, and 72 h after co-culture （ all P 〈 0.05 ）. Conclusion The placental mesenchymal stem cell modified by HLA-G gene can in hibit the proliferation of CD4＋ T lymphocytes.

关 键 词：胎盘间充质干细胞 人类白细胞抗原G T淋巴细胞 细胞增殖 

分 类 号：R458[医药卫生—治疗学]