基于稀土纳米颗粒的切伦科夫激发荧光断层显像  

Cerenkov luminescence excited fluorescence tomography based on rare earth nanoparticles

作　　者：高永恒[1] 马晓伟[1] 曹旭 康飞[1] 王政杰[1] 杨卫东[1] 汪静[1] 

机构地区：[1]第四军医大学附属西京医院核医学科,西安710032 [2]西安电子科技大学生命科学技术学院,710071

出　　处：《中华核医学与分子影像杂志》2018年第2期104-107,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金（81230033）

摘　　要：目的利用稀土纳米颗粒（RENPs）的切伦科夫能量转移效应对切伦科夫光信号进行增强得到切伦科夫激发荧光信号，比较切伦科夫激发荧光断层显像（CLFT）和切伦科夫断层显像（CLT）的定位准确性。方法在体外用68Ga（0．74MBq）分别激发Y2O3：Eu^3＋、Er2O3及Eu2O3（质量浓度均为10mg／m1），并与68Ga产生的切伦科夫光信号进行对比；用不同活度（3．70、1．85、0．92、0．46、0．23MBq）的68Ga分别激发10mg／ml的Y2O3：Eu^3＋，再用3．70MBq的68Ga分别激发不同质量浓度（10．0、5．0、2．5、1．2、0．6mg／m1）的Y2O3：Eu^3＋，以观察增强光信号强度与核素活度及Y2O3：Eu^2＋质量浓度的关系。将含0．74MBq68Ga以及0．74MBq。Ga和1mgY2O3：Eu^3＋溶液的聚乙烯管分别植入2只裸鼠体内，先后行PET／CT及光学显像，并行光学三维重建。采用单因素方差分析、两样本t检验和直线相关分析进行数据比较。结果Y2O3：Eu^3＋可以显著且稳定地增强切伦科夫光信号（F=53．35，q=17．03，P〈0．001）；增强光信号的强度在一定范围内分别与68Ga活度（r=0．99）及Y2O3：Eu^3＋浓度（r=0．93）均呈正相关。三维重建结果显示，CLFT比CLT具有更高的光源位置相似度（0．43±0．14和0．16±0．06；f=5．090，P〈0．05）。结论相比于CLT，基于Y2O3：Eu^3＋的CLFT能够更准确地反映放射性核素药物的空间分布，提示CLVF在生物光学成像中更具潜力。Objective Using Cerenkov radiation energy transfer （CRET） effect of rare earth nano- particles （RENPs） to enhance and convert Cerenkov luminescence into Cerenkov luminescence excited fluo- rescence, and to compare the accuracy of Cerenkov luminescence tomography （CLT） and Cerenkov lumi- nescence excited fluorescence tomography （CLFT）. Methods 68Ga （0.74 MBq） was used to respectively excite Y203 ：Eu3＋, Er203 and Eu203（ 10 mg/ml）. Various radioactivities of 68Ga （3.70, 1.85, 0.92, 0.46, 0.23 MBq） were used to respectively excite Y203 ：Eu3＋ with a fixed concentration （ 10 mg/nfi）. A fixed radio- activity of 68Ga （3.70 MBq） was used to excite Y203 ：Eu3＋ with different concentrations （10.0, 5.0, 2. 5, 1.2, 0.6 mg/ml） in order to find the relationships between the optical intensity and the radioactivity of 68Ga or the concentration of Y2O3 ：Eu3＋. Polyethylene tubes containing 68Ga （0.74 MBq） and 68Ga （0.74 MBq） ＋ Y2 03 ：Eu3＋（ 1 mg） were respectively implanted into two nude mice, then PET/CT and optical imaging were acquired. Three-dimensional reconstruction was proceeded. One-way analysis of variance, two-sample t test, linear correlation analysis were used for data analysis. Results Y203 ：Eu3＋ could significantly and stably enhance the Cerenkov optical signal （F= 53.35, q= 17.03, P〈0.001 ）. The enhanced optical signal intensi- ty had linear relationships with the radioactivity of 68Ga or the concentration of Y203 ：Eu3＋（ r values： 0. 99 and 0.93 ）. Three-dimensional reconstruction result showed that CLFT had significantly higher similarity than CLT （0.43±0.14 vs 0. 16±0.06, t=5.090, P〈0.05）. Conclusion CLFT could reflect the distribution of ra- diopharmaceuticals more precisely than CLT, and therefore might have potential in biologic optical imaging.

关 键 词：稀土元素 纳米粒 光学成像 切伦科夫辐射 

分 类 号：R817.4[医药卫生—影像医学与核医学]