鸭瘟病毒强、弱毒株TK基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis on the TK Gene of DPV′s Virulent and Vaccine Strain

作　　者：任攀 刘情[1] 李文文[1] 刘亚刚[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《四川畜牧兽医》2018年第2期31-33,共3页Sichuan Animal & Veterinary Sciences

基　　金：国家农业科技成果转化资金项目--国家科技部农业项目"规模化养鸭场标准化健康养殖技术示范与推广"(2013GB2F000422)

摘　　要：本研究根据Gen Bank上发表的鸭瘟病毒(DPV)基因序列设计了1对引物,分别对DPV AV 1221株和DPV鸡胚化弱毒疫苗株的TK基因进行扩增,扩增的目的片段克隆至载体p MD19-T,经PCR和双酶切(Bam HⅠ+HindⅢ)鉴定,将阳性质粒进行测序和生物信息学分析。结果成功克隆出了长度均为1 077 bp的DPV TK基因,生物信息学分析结果表明两个片段的同源性达到100%。According to the sequence of TK gene in GenBank, a pair of primers was designed to amplify this gene in virulent and vaccine duck plague virus strains. The purpose amplified fragment was cloned into pMD 19-T vector. The recombinant expression vector was selected and identified by PCR and restriction enzyme digestion （BarnH I ＋Hind llI ）. The positive recombinant plasmid was then sequenced and the biological information was also analyzed. The results showed this research successfully cloned the DPV TK gene, and both of the fragments were 1 077 bp, the result of bioinformaties analysis revealed the ho- mology of two fragments up to 100%.

关 键 词：鸭瘟病毒 TK基因 TK蛋白 生物信息学分析 

分 类 号：S858.322.659.1[农业科学—临床兽医学]