饮用水中氨氮测定的水杨酸盐光度法的改进  

Improvement of salicylates spectrometry for determination of ammonia-nitrogen in drinking water

作　　者：陈翔[1] 倪蓉[1] 张燕[1] 刘兰侠[1] 

机构地区：[1]上海市徐汇区疾病预防控制中心,上海200237

出　　处：《现代预防医学》2018年第3期510-512,共3页Modern Preventive Medicine

摘　　要：目的提高氨氮的分析速度并扩大方法的线性范围。方法使用空气浴加快显色速度,改用5 mm比色皿比色,使用酒石酸钾钠作为掩蔽剂。结果最大吸收波长为700nm,空气浴显色条件为40℃,20 min,摩尔吸光系数为1.25×10~4L/(mol·cm)。线性范围为0.035-2.5mg/L,检出限和定量限分别为0.010mg/L和0.035mg/L,氨氮浓度为0.044mg/L^2.02mg/L的5种水样,其RSD为1.84%~0.58%,在2个不同添加水平下,3种水样的回收率在98.7%~100%间,质控样测定结果均在标准范围内,和国标法测定结果无显著差异。结论改进后的方法显著提高了氨氮检测速度,有效扩展了原法的线性范围。Objective To accelerate the speed of determination of ammonia nitrogen and enlarge the linear range of the method. Methods The color reaction was accelerated by air - bath. The length of the colorimetric cell was changed to 5 mm and the potassium sodium tartrate was used as a masking agent. Results The wavelength of the maximum absorption peak was 700 nm. The color reaction conditions of the air bath were 40℃ and 20 minutes. The molar absorption coefficient was 1.25 × 10^4 L/（ mol · cm）. The linearity was from 0.035 to 2.5 mg/L. The LOD and LOQ were 0. 010 mg/L and 0. 035 mg/L,respectively. The relative standard deviation was between 1.84% and 0.58% for 5 water samples with the NH3 - N concentration from 0. 044 mg/L to 2.02 mg/L. The recoveries of 3 spiked water samples were between 98.7% and 100% at two spiking levels. The results of 5 quality control water samples were all within the given values. There was no significant difference between the improved method and the national standard method. Conclusion The speed of ammonia nitrogen determination is accelerated distinctly by the improved method and the linearity range is enlarged effectively.

关 键 词：氨氮 水杨酸盐光度法 饮用水 

分 类 号：R115[医药卫生—公共卫生与预防医学]