染料木黄酮对高能日粮肉鸡胆固醇代谢的影响  

Effects of Genistein on Cholesterol Metabolism in Broilers Fed a High-Energy Diet

作　　者：陈咏然 张明 田宁 朱力 杨鹰[1] 

机构地区：[1]动物营养学国家重点实验室,中国农业大学动物科技学院,北京100193

出　　处：《中国畜牧杂志》2018年第2期93-98,共6页Chinese Journal of Animal Science

基　　金：国家自然科学基金项目(31372327)

摘　　要：为研究染料木黄酮(Gen)对高能日粮肉鸡胆固醇代谢的影响,试验选用11日龄AA肉鸡300只,随机分成5组,每组6个重复,每个重复10只。对照组饲喂基础日粮,2个豆油高能组日粮中增加豆油(5.6%,11~21 d)和(6.6%,22~42 d)使代谢能提高5%,其中1个豆油组添加50 mg/kg Gen;2个猪油高能组增加猪油(5.1%,11~21 d)和(6.2%,22~42 d)使日粮代谢能提高5%,其中1个猪油组日粮中添加50 mg/kg Gen,试验期31 d。结果表明:日粮中添加Gen后豆油高能日粮诱导的肝脏总胆固醇(TC)和血清甘油三酯(TG)显著降低,豆油和猪油高能日粮诱导的血清脂蛋白脂酶(LPL)显著降低(P<0.05);添加50 mg/kg Gen可显著降低高能日粮处理组肉鸡肝脏3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)的活性及蛋白含量,同时降低固醇调节元件结合蛋白-2(SREBP-2)含量(P<0.05);高能日粮提高了HMGCR基因的表达(P<0.05),对CYP7A1、SREBP-2等基因的表达无显著影响(P>0.05);而Gen可降低高能日粮肉鸡肝脏中上述基因的表达。在本试验条件下,高能日粮中添加50 mg/kg Gen可降低肉鸡血脂及肝脏胆固醇的合成和沉积。To investigate the effects of genistein on cholesterol metabolism in broilers fed a high-energy diet. A total of 300 eleven-day-old health AA male broilers were used for a 31-day experiment. Chicks were randomly assigned into 5 treatments with 6 replicates of 10 males in each group. Chickens in the control group were fed soybean-corn based starter or finisher diets to meet nutrient requirements. Chickens in high energy treatment groups were fed with a high energy with 5% metabolism energy （provided by soybean oil or lard oil） more than the NRC standard from day 11-42 in the presence or absence of genistein （50 mg/kg）. Chickens in treatment I and II were fed with soybean oil （5.6%, 11-21 d） or （6.6%, 22-42d） in the presence or absence of genistein （50 mg/kg）. Chickens in treatment In and IV were fed with lard oil （5.1%, 11-21 d） or （6.2%, 22-42d） in the presence or absence of genistein （50 mg/kg）. Biochemical analysis showed that high energy density diet induced higher total cholesterol （TC）, total glycerol （TG）, and lipoprotein lipase （LPL） in serum were significantly abolished by genistein （P 〈0.05）. Genistein tended to increase the high-density lipoproteincholesterol （HDL-C） concentration in the serum. Additionally, high energy density diet induced higher TC in liver was significantly diminished by genisein （P〈0.05）. High energy density diet induced a pronounced increase in activity of 3-hydroxy-3-methylglutaryl-CoA reductase （HMGCR） （P〈0.05）. And these effects were significantly diminished by genisein （P〈0.05）. Genistein （50 mg/kg） significant reduced the content of SREBP-2 （P〈0.05）. The mRNA level of liver 3-hydroxy-3-methylglutaryl-CoA reductase was upregulated by high energy diet, which was markedly reversed by genistein （P〈0.05）. Energy density had no effect on the expression of CYP7A1 and SREBP-2. However, the expression of CYP7A1 and SREBP-2 were markedly decreased by genistein （P〈0.05）. In conclusion, 50mg/

关 键 词：染料木黄酮 高能日粮 肉鸡 血清 肝脏 胆固醇代谢 

分 类 号：S831.5[农业科学—畜牧学]