Single-molecule imaging and tracking of molecular dynamics in living cells  

作　　者：Nan Li Rong Zhao Yahong Sun Zi Ye Kangmin He Xiaohong Fang 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Molecular Nanostructure and Nanotechnology,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Cell Biology,Harvard Medical School,and Cellular and Molecular Medicine Program,Boston Children’s Hospital,Boston,MA 02115,USA

出　　处：《National Science Review》2017年第5期739-760,共22页国家科学评论（英文版）

基　　金：supported by National Basic Research Program of China(2013CB933701);National Natural Science Foundation of China(21127901,91413119,91213305);Chinese Academy of Sciences

摘　　要：Unlike the ensemble-averaging measurements, the single-molecule imaging and tracking(SMIT) in living cells provides the real-time quantitative information about the locations, kinetics, dynamics and interactions of individual molecules in their native environments with high spatiotemporal resolution and minimal perturbation. The past decade has witnessed a transforming development in the methods of SMIT with living cells, including fluorescent probes, labeling strategies, fluorescence microscopy, and detection and tracking algorithms. In this review, we will discuss these aspects with a particular focus on their recent advancements. We will then describe representative single-molecule studies to illustrate how the single-molecule approaches can be applied to monitor biomolecular interaction/reaction dynamics, and extract the molecular mechanistic information for different cellular systems.Unlike the ensemble-averaging measurements, the single-molecule imaging and tracking （SMIT） in living cells provides the real-time quantitative information about the locations, kinetics, dynamics and interactions of individual molecules in their native environments with high spatiotemporal resolution and minimal perturbation. The past decade has witnessed a transforming development in the methods of SMIT with living cells, including fluorescent probes, labeling strategies, fluorescence microscopy, and detection and tracking algorithms. In this review, we will discuss these aspects with a particular focus on their recent advancements. We will then describe representative single-molecule studies to illustrate how the single-molecule approaches can be applied to monitor biomolecular interaction/reaction dynamics, and extract the molecular mechanistic information for different cellular systems.

关 键 词：single-molecule imaging single-molecule tracking live-cell imaging fluorescence microscopy quantitative analysis 

分 类 号：Q23[生物学—细胞生物学]