运动训练对高血压模型大鼠心脏功能的影响  

Effects of exercise training on cardiac function in hypertensive rats

作　　者：朴艳虹 刘文博[1] 金春子[1,2] 崔兰[1] 

机构地区：[1]延边大学附属医院心内科 [2]延边大学附属医院中心实验室,吉林延吉133000

出　　处：《延边大学医学学报》2017年第3期160-163,共4页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金(81460039;81760075);吉林省卫生厅青年科研基金(2014Q043)

摘　　要：[目的]探讨运动训练对高血压模型大鼠心脏功能的影响.[方法]取健康雄性SD大鼠,随机分为非运动对照组(Sham)、运动组(Sham-ET)、高血压组(Ang-HT)和高血压运动组(Ang-ET).利用血管紧张素Ⅱ诱导建立大鼠高血压模型.采用多动物多通道尾袖套法无创血压系统监测大鼠血压值,心脏超声仪测定左心室射血分数(LVEF).分离心肌细胞,采用IonOptix单细胞收缩检测系统检测心肌细胞收缩性及肌丝钙敏感性.[结果]运动2周末(制作模型4周末)时,Ang-HT与Ang-ET组收缩压和舒张压间差异均有统计学意义(P<0.05),Sham与Sham-ET组收缩压与舒张压间差异均无统计学意义(P>0.05),Ang-HT与Ang-ET组LVEF及心肌细胞收缩性间差异均有统计学意义(P<0.05),Ang-ET组EC50值较Ang-HT组明显缩短(P<0.05).[结论]运动训练通过恢复心肌细胞肌丝钙离子敏感性而改善心肌细胞收缩性及心功能.OBJECTIVE To investigate the effects of exercise training on cardiac function in hypertensive rats.METHODS Male SD rats were randomly divided into four groups：sham operation group（Sham）,sham operation exercise group（Sham-ET）,hypertension group（Ang-HT）and hypertension exercise group（Ang-ET）.Angiotensin Ⅱ was used to establish the hypertension rat model.Noninvasive blood pressure multiple animal tail cuff technique was used to monitor rat blood pressure.The left ventricular ejection fraction（LVEF）was detected by cardiac ultrasonography.Myocardial cells were isolated,and IonOptix single cell shrinkage detection system was used to detect myocardial cell contractility and myofilament calcium sensitivity.RESULTS After 2 weeks exercise training（4 weeks modelling）,the difference between systolic and diastolic pressure in the Ang-HT and Ang-ET group was statistically significant（P0.05）,while there was no statistically significant difference between the Sham and Sham-ET group（P0.05）;The differences of LVEF and myocardial cell contractility in Ang-HT and Ang-ET group had statistical significance（P0.05）,and the value of EC50 in the Ang-ET group was obviously decreased than that in the Ang-HT group（P0.05）.CONCLUSION Exercise training could improve myocardial cell contractility and cardiac function by restoring the myofilament calcium sensitivity.

关 键 词：左室射血分数 心肌细胞收缩性 运动训练 高血压 肌丝钙敏感性 

分 类 号：R544.1[医药卫生—心血管疾病]