非浓缩尿蛋白电泳在原发性高血压肾损伤中的应用评价  

The Evaluation of Applicationof SDS-AGE of Non-concentrated Proteinuria Electrophoresis in Essential Hypertension Renal Injury

作　　者：王加 王珏 

机构地区：[1]南京医科大学第一附属医院医学检验学部,江苏南京210029

出　　处：《标记免疫分析与临床》2018年第1期126-129,共4页Labeled Immunoassays and Clinical Medicine

基　　金：江苏省实验诊断学重点实验室基金(编号:XK 201114)

摘　　要：目的探讨十二烷基磺酸钠-琼脂糖凝胶(Sodium dodecyl sulphat-agarose gel electrophoresis,SDS-AGE)非浓缩尿蛋白电泳在原发性高血压肾损伤中的诊断价值。方法收集2016年3月~2016年8月在南京医科大学第一附属医院就诊的原发性高血压患者57例,根据尿蛋白定性结果进行分组,同时选取同期健康体检者20例作为健康对照组,进行SDS-AGE非浓缩尿蛋白电泳、24h尿蛋白(24h urine protein,24h Upro)、血清肌酐(serum creatinine,SCr)和尿素氮(blood urea nitrogen,BUN)水平测定,多样本总体均数间的比较采用LSD-t检验。结果 57例原发性高血压患者中见病理性蛋白尿30例(30/57,52.6%)。尿蛋白(+/-)组见肾小管性蛋白尿2例(2/5,40%),尿蛋白(1+)组和尿蛋白(2+)组均见各种类型蛋白尿,尿蛋白(3+)组见肾小球性蛋白尿和混合性蛋白尿。在不同类型蛋白尿的24h Upro、SCr和BUN定量比较中发现,单纯白蛋白性、肾小球性、肾小管性和混合性这四种类型蛋白尿24h Upro的水平有明显差异,差异有统计学意义(P<0.05)。而SCr和BUN的检测只有混合性蛋白尿类型明显高于其余三种类型,差异有统计学意义(P<0.05)。结论非浓缩尿蛋白电泳技术可直观反映尿蛋白组分,判断肾脏损伤部位,更早地提示原发性高血压肾功能的改变。Objective To study the diagnostic value of SDS- AGE of non- concentrated proteinuria electrophoresis in essential hypertension renal injury. Methods 57 patients with essential hypertension were selected and divided according to the qualitation results of urine protein. SDS-AGE electrophoresis, 24h Upro, SCr and BUN were performed and detected. LSD- t test was used for the analysis. Results In 57 cases essential hypertension patients, there were 30 cases of pothological proteinuria which includes 2 cases of tubular proteinuria in Pro （ ＋/- ） group, all types of proteinuria in Pro （ ＋ ） and Pro （2 ＋ ） group, glomerular and mixed proteinuria in Pro （ 3 ＋ ） group. There were statistical significant differences between each group including simple, glomerular, tubular and mixed proteinuria in the levels of 24h Upro（ P 〈0.05 ）. Conclusion SDS-AGE of non-concentrated proteinufia electrophoresis can detect urinary protein components, and assess the position of kidney damage, which reflect the renal injury in essential hypertension earlier.

关 键 词：SDS-AGE电泳 原发性高血压 肾损伤 

分 类 号：R544.11[医药卫生—心血管疾病] R692[医药卫生—内科学]