肝缺血再灌注损伤对大鼠心肾能量代谢的研究  

Study of hepatic ischemia-reperfusion injury on cardiac and renal energy metabolism in rats

作　　者：黎明[1] 巴图德力根[1] 马凤艳[2] 那日苏[2] 韩志强[1] 

机构地区：[1]内蒙古民族大学附属医院,内蒙古通辽028007 [2]内蒙古民族大学蒙医药学院,内蒙古通辽028000

出　　处：《中国临床药理学杂志》2018年第3期275-278,共4页The Chinese Journal of Clinical Pharmacology

基　　金：内蒙古自治区医疗卫生科研计划基金资助项目(201302120)

摘　　要：目的研究肝缺血再灌注损伤后对心肾能量代谢指标的影响。方法按照体重将大鼠随机分为5组,每组10只:假手术组、模型-0 h组、模型-2 h组、模型-4 h组、模型-6 h组。用无损伤血管夹钳夹后造成缺血再灌注损伤模型。按再灌注后即刻和再灌注后2,4,6 h,立即处死动物,取材。用酶联免疫吸附法检测组织的钠钾ATP酶、钙镁ATP酶含量。结果假手术组、模型-2 h组、模型-4 h组的肝组织Ca^(2+)-Mg^(2+)-ATP酶含量分别为(343.84±22.11),(221.88±64.15),(235.02±58.84)μmol·g^(-1),与假手术组比较,模型-2 h、4 h组的差异均有统计学意义(均P<0.05)。假手术组、模型-2 h、4 h组的肾组织Na^+-K^+-ATP酶含量分别为(79.46±10.84),(67.28±3.39),(69.09±1.47)μmol·g^(-1);这3组的Ca^(2+)-Mg^(2+)-ATP酶含量分别为(345.68±21.68),(227.39±50.63),(246.38±64.31)μmol·g^(-1),与假手术组比较,这3组差异均有统计学意义(均P<0.05)。假手术组和模型-0,2,4,6 h组的心肌组织Na^+-K^+-ATP酶含量分别为(68.16±12.17),(71.92±9.91),(61.62±7.96),(59.23±3.86),(79.72±9.91)μmol·g^(-1);这5组的Ca^(2+)-Mg^(2+)-ATP酶含量分别为(261.05±40.52),(279.13±34.12),(243.62±23.59),(244.66±17.79),(291.78±36.38)μmol·g^(-1),与假手术组比较,这5组的差异均无统计学意义(均P>0.05),表明心肌组织能量代谢指标无明显变化。结论肝缺血再灌注损伤时,心、肾功能同时受损,但肾损伤早于心肌损伤。Objective To study the influence of hepatic ischemia-reperfusion injury（ IRI） on cardiac and renal energy metabolism in rats.Methods The rats were randomly divided into five groups： the sham operation group,model-0 h group,model-2 h group,model-4 h group,model-6 h group,and ten rats in each group. The hepatic IRI rats model was prepared with non traumatic vascular clamp. The animal were sacrificed immediately after IRI 0,2,4,6 h and samples were collected. The tissue content of Na~＋-K~＋-ATPase and Ca^（2＋）-Mg^（2＋）-ATPase were detected by enzyme linked immunosorbent assay. Results The Ca^（2＋）-Mg^（2＋）-ATPenzyme of liver tissue in sham operation group,model-2 h group,model-4 h group were（ 343. 84 ± 22. 11）,（ 221. 88 ± 64. 15）,（ 235. 02 ± 58. 84） μmol·g^（-1）; compared with the sham operated group,the differences were statistically significant（ all P〈 0. 05）. The Na~＋-K~＋-ATP enzyme of renal tissuein shamoperation group,model-2 h group,model-4 h group were（ 79. 46 ± 10. 84）,（ 67. 28 ± 3. 39）,（ 69. 09 ± 1. 47）μmol·g^（-1）. The Ca^（2＋）-Mg^（2＋）-ATP enzyme of renal tissue in the three groups were（ 345. 68 ± 21. 68）,（ 227. 39 ± 50. 63）,（ 246. 38 ± 64. 31） μmol·g^（-1）; compared with the sham operated group,the difference was statistically significant（ all P〈 0. 05）. The Na~＋-K~＋-ATP enzyme of myocardium tissue in sham operation group,model-0 h group,model-2 h group,model-4 h group,model-6 h groupwere（ 68. 16 ± 12. 17）,（ 71. 92 ± 9. 91）,（ 61. 62 ± 7. 96）,（ 59. 23 ± 3. 86）,（ 79. 72 ± 9. 91） μmol·g^（-1）. The Ca^（2＋）-Mg^（2＋）-ATP enzyme of myocardium tissue in the five groups were（ 261. 05 ± 40. 52）,（ 279. 13 ± 34. 12）,（ 243. 62 ± 23. 59）,（ 244. 66 ± 17. 79）,（ 291. 78 ± 36. 38） μmol·g^（-1）; compared with the sham operated group,the differences were statistically significant（ all P 〈0. 05）. There were no obvious changes in myo

关 键 词：蒙医五行 肾、肝、心的母子关系 肝缺血再灌注 能量代谢 

分 类 号：R97[医药卫生—药品]