薏苡茎叶提取物石油醚部位的体外抗肿瘤活性研究  

Study on the antitumor activity of petroleum ether fractions of the extract from stems and leaves of Coix in vitro

作　　者：林瑶 陆世惠[1,2] 喻巧容 黄锁义[1,2] 

机构地区：[1]右江民族医学院药学院,广西百色533000 [2]右江民族医学院广西高校右江流域特色民族药研究重点实验室,广西百色533000 [3]广西中医药大学药学院,南宁530001

出　　处：《中国临床药理学杂志》2018年第3期282-284,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81360684);广西自然科学基金资助项目(2011GXNSFA018046);广西中医药科技专项基金资助项目(GZKZ10-128);广西教育厅课题基金资助项目(201106LX425);广西中医药管理局基金资助项目(gzzc1047);广西医学科学实验中心开放基金专项资助项目(KFJJ2011-04);广西高校科学技术研究基金资助项目(KY2015LX291)

摘　　要：目的探讨薏苡茎、叶提取液的石油醚萃取部位的体外抗肿瘤作用。方法用CCK-8法检测薏苡茎、叶不同萃取液对3种肿瘤细胞株体外生长的抑制作用。结果薏苡茎、叶4种提取液对所试肿瘤细胞株的体外生长均有抑制作用,其中薏苡茎的水提物石油醚萃取液对人宫颈癌(HeLa)和薏苡茎的醇提物石油醚萃取液对胃癌(SGC-7901)的半数抑制浓度(IC_(50))分别为51.78和74.44μg·mL^(-1),薏苡叶的水提物石油醚萃取液对HeLa和薏苡叶的醇提物石油醚萃取液对SGC-7901的IC_(50)分别为45.22和45.91μg·mL^(-1)。结论薏苡茎、叶提取液的石油醚萃取部位具有较好的抗肿瘤活性。Objective To explore the anticancer effect of petroleum ether fraction of the extract from stems and leaves of Coix lacryma in vitro. Methods The in vitro growth inhibitory effects of different solutions of the extract of Coix stems and leaves on 3 kinds of tumor cell lines was detected by CCK-8 assay. Results Four kinds of Coix extract can inhibit the growth of the tested tumor cell lines in vitro. For the extracts of Coix stems,the half maximal inhibitory concentrations（ IC_（50））of petroleum ether fractions of the water extract on HeLa and ethanol extract on SGC-7901 were 51. 78 and 74. 44 μg·mL^（-1）. And,for the extracts of Coix leaves, they were 45. 22 and 45. 91 μg·mL^（-1）.Conclusion The petroleum ether fractions of the extract of Coix stems and leaves had better anti-tumor activity.

关 键 词：薏苡茎叶 CCK-8 体外 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]