利拉鲁肽对高脂喂养肥胖大鼠胰腺中核转录因子κB和蛋白酪氨酸磷酸酶1B的影响  

Effects of liraglutide on expression of nuclear factor-kappa B and protein tyrosine phosphatase-1B in pancreas of the obese rats induced by high fat diet

作　　者：刘洋[1] 赵琳[1] 

机构地区：[1]解放军第309医院内分泌科,北京100093

出　　处：《中国临床药理学杂志》2018年第3期294-296,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究利拉鲁肽对高脂喂养肥胖大鼠胰腺中核转录因子κB(NF-κB)和蛋白酪氨酸磷酸酶1B(PTP-1B)表达的影响。方法用高脂饮食诱导建立大鼠肥胖模型,并随机分组对照组和实验组,每组7只;另取7只正常大鼠作为空白组。实验组予以皮下注射利拉鲁肽注射液0.6 mg·kg^(-1)·d^(-1),对照组和空白组均予以皮下注射同体积0.9%NaCl约2 mL。3组大鼠均干预2周。比较3组大鼠胰腺中NF-κB活性和PTP-1B蛋白表达量。结果干预2周后,实验组、对照组和空白组的NF-κB积分灰度值分别为69.2,150.5和70.4,PTP-1B蛋白表达量分别为(1.53±0.34),(1.79±0.12)和(1.32±0.34),实验组和空白组的上述指标比较,差异均无统计学意义(均P>0.05),对照组的上述指标与实验组、空白组比较,差异均有统计学意义(均P<0.05)。结论利拉鲁肽注射液可阻断高脂饮食对胰腺组织NF-κB的活化作用,同时可降低PTP-1B蛋白的表达。Objective To observe the impact of liraglutide on expression of nuclear factor-kappa B（ NF-κB） and protein tyrosine phosphatase-1 B（ PTP-1 B） in pancreas of the obese rats induced by high fat diet.Methods Obese rats model induced by high-fat-diet were randomly divided into control group（ n = 7 cases） and test group（ n = 7 cases）.Another 7 normal rats were assigned to blank group. After modeling,test group received subcutaneous injection of liraglutide 0. 6 mg·kg^（-1）·d^（-1） for 2 weeks. Control and blank groups took 2 mL subcutaneous injection of 0. 9% NaCl. We observed the expression and activity of NF-κB and PTP-1 B in pancreas in three groups. Results After 2 weeks exposure,the activity of NF-κB in test,control and blank groups were 69. 2,150. 5 and 70. 4,the expression of PTP-1 B in test,control and blank groups were（ 1. 53 ± 0. 34）,（ 1. 79 ± 0. 12） and（ 1. 32 ± 0. 34）. The differences were statistically significant between blank group and test or blank groups（ all P〈 0. 05）,and the differences were not significant between test group and blank group（ all P〉 0. 05）. Conclusion Liraglutide injection can block the activation of NF-κB induced by high fat diet in pancreatic tissue thus reducing the expression of PTP-1 B,whichimplied that liraglutide might has been closely involved in the signal transduction pathway of NF-κB.

关 键 词：利拉鲁肽注射液 胰岛素抵抗 蛋白酪氨酸磷酸酶1B 凝胶电泳迁移率变化分析 

分 类 号：R977.15[医药卫生—药品]