海南岛海菖蒲种群克隆多样性和遗传结构  

Clonal diversity and genetic structure of Enhalus acoroides populations along Hainan Island,China

作　　者：蒋凯[1,2] 高辉 陈小勇[1] 

机构地区：[1]华东师范大学生态与环境科学学院/上海市城市化生态过程与生态恢复重点实验室,上海200241 [2]上海辰山植物园/中国科学院上海辰山植物科学研究中心,上海201602

出　　处：《应用生态学报》2018年第2期397-402,共6页Chinese Journal of Applied Ecology

基　　金：科技部科技基础性工作专项(2014FY130300)资助~~

摘　　要：海草是生长在潮间带和潮下带的单子叶植物,由海草植物组成的海草床是生态系统服务价值最高的生态系统之一.然而,近几十年人类活动干扰、全球气候变化等因素导致海草床衰退严重.海菖蒲是分布于热带、体型最大的雌雄异株海草,我国位于该物种的分布北缘,本文对其克隆多样性和遗传结构进行研究,以期为该海草的保护提供参考.采用4对多态微卫星标记对采自海南岛4个地点的现存海菖蒲种群的样品进行基因型分型.结果表明:海菖蒲种群克隆多样性和遗传多样性较低,这与所研究种群处于分布区北缘有关;种群间遗传分化值范围较大(0.073~0.309),这可能是由于分布于不同港湾的种群间距离范围较大以及局域绝灭/再拓殖的遗传漂变效应所致;各种群未发现近期经历种群瓶颈的信号,很可能是由于种群内遗传多样性已经很低,种群减小未能导致遗传多样性明显降低.根据种群遗传特征,提出了重点保护种群的建议,鉴于目前我国海菖蒲等海草快速衰退的局面,应强化海草保护并实施海草床生态恢复.Seagrasses are monocotyledons that grow in intertidal and subtidal zones. Seagrass beds are among ecosystems with the highest ecosystem service value. However, seagrass beds are rapidly decline due to anthropogenic disturbances and global climate change. Enhalus acoroides, a monoecious perennial species, is the largest seagrass in stature. It distributes in tropical regions, with Hainan Island as its northern limits. Clonal diversity and genetic structure of E. acoroides populations in Hainan Island were studied to facilitate its conservation and restoration. We used four polymorphic microsatellites to genotype samples collected from four extant populations. We found low clonal and genetic diversities within populations, consistent with the expectations of genetic structure in edge populations. A large range of genetic differentiation was found between these four populations, probably due to the large range of geographic distances between them and genetic drift by local extinction and recolonization. We found no sign of recent bottlenecks in all the populations, probably due to the within-population genetic diversity being too low to show obvious reduction even after bottlenecks. We proposed suggestions on population conservation for those with high priority. Given that seagrass bed had experienced rapid decline in recent years, strengthened conservation and ecological restoration are urgently needed.

关 键 词：海菖蒲 微卫星 克隆多样性 遗传多样性 遗传分化 

分 类 号：Q943[生物学—植物学]