氟苯尼考对嗜水气单胞菌的防突变浓度  

Determination of mutant prevention concentration of florfenicol against Aeromonas hydrophilia

作　　者：单奇[1] 刘书贵[1] 马丽莎[1] 李丽春[1] 郑光明[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部休闲渔业重点实验室,农业部水产品质量安全风险评估实验室(广州),广东广州510380

出　　处：《中国渔业质量与标准》2018年第1期55-60,共6页Chinese Fishery Quality and Standards

基　　金：国家自然科学基金(31502125);广东省自然科学基金-博士启动(2015A030310086);农业部水产品质量安全风险评估(GJFP201501001)

摘　　要：通过测定氟苯尼考对嗜水气单胞菌的最低抑菌浓度(MIC)和防突变浓度(MPC),为水产养殖中合理使用该药提供基础数据。采用琼脂二倍稀释法测定氟苯尼考对嗜水气单胞菌的MIC和MIC_(99)(抑制99%细菌生长的最低抑菌浓度);肉汤法富集嗜水气单胞菌后,采用琼脂稀释法测定氟苯尼考对嗜水气单胞菌的暂定防突变浓度(MPC_(pr))和MPC,并计算选择指数SI(即MPC/MIC_(99))。结果显示,氟苯尼考对嗜水气单胞菌ATCC7966的MIC为1.0μg/m L,MIC_(99)为0.8μg/m L;MPC为4.8μg/m L;SI(MPC/MIC_(99))为6;突变选择窗(MSW)(MIC_(99)~MPC)为0.8~4.8μg/m L。氟苯尼考对分离于患病水生动物的40株嗜水气单胞菌的MIC_(50)、MIC_(90)和MPC_(90)分别为1.0、2.0和16.0μg/m L;SI(MPC_(90)/MIC_(90))为8。结果表明,氟苯尼考对嗜水气单胞菌的MPC90较高,易诱导嗜水气单胞菌产生耐药性。出于对药物安全性问题的考虑,建议氟苯尼考与其他抗菌药物联合使用,或是缩小甚至关闭耐药突变选择窗的最佳选择。The minimal inhibitory concentration( MIC) and mutant prevention concentration( MPC) of florfenicol against Aeromonas hydrophilia were investigated in order to provide the basic data for rationally using the drug in aquaculture. Two-fold serial dilution method of agar was adopted to determine MIC and MIC_(99) of florfenicol against A. hydrophilia. After enriching A. hydrophilia strains in broth,the provisional mutant prevention concentration( MPC_(pr))and MPC of florfenicol were determined by agar plate dilution method,and the selection index( SI) was also calculated. The MIC,MIC_(99),MPC and MPC/MIC_(99) of florfenicol against A. hydrophilia strain ATCC 7966 were 1. 0,0. 8,4. 8 and 6 μg/m L,respectively. The mutant selection window( MSW,MIC_(99)-MPC) was 0. 8-4. 8 μg/m L. The MIC_(50),MIC_(90),MPC_(90) and MPC_(90)/MIC_(90) of florfenicol against A. hydrophilia clinically isolated from diseased aquatic animals were 1. 0,2. 0,16. 0 μg/m L and 8 respectively. These results showed that florfenicol had a higher MPC_(90) against A. hydrophilia,which could easily induce A. hydrophilia drug-resistance. Considering drug safety,the best option was suggested by combining with other antibiotics,or reducing even closing mutant selection window( MSW).

关 键 词：防突变浓度 氟苯尼考 嗜水气单胞菌 选择指数 耐药突变选择窗 

分 类 号：S91[农业科学—水产科学]