南极菌株SC-5同步糖化发酵甜高粱秆生产纤维素乙醇的研究  

Study on Simultaneous Saccharification and Fermentation of Sweet Sorghum Stalk for Cellulose Ethanol Production with Antarctic Strain SC-5

作　　者：马德源[1,2] 范仲学 郭凤丹[1,2] 王能飞 王颖[4] 张斌 

机构地区：[1]山东省农业科学院生物技术研究中心,山东济南250100 [2]山东省作物遗传改良与生态生理重点实验室,山东济南250100 [3]国家海洋局第一海洋研究所,山东青岛266061 [4]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《山东农业科学》2018年第2期139-142,共4页Shandong Agricultural Sciences

基　　金：山东省重点研发计划项目(2015GNC113011)

摘　　要：利用从南极土壤中分离筛选的产低温纤维素酶菌株SC-5,以甜高粱秆为发酵底物,开展同步糖化发酵生产纤维素乙醇试验研究。结果表明:SC-5低温纤维素酶,底物亲合性好,最适pH值为6.0,最适作用温度为40℃,pH适应性广,热稳定性好,乙醇耐受性好。同步发酵1.0 kg甜高粱秆可生产45 g乙醇,乙醇生产效率较高,发酵工艺简单实用。The strain SC-5 producing low temperature cellulase was isolated from the Antarctic soil.With the sweet sorghum stalks as substrate,the experiment was carried out for simultaneous saccharification and fermentation to produce cellulosic ethanol using SC-5. The results showed that the SC-5 strain had stronger ability to produce low-temperature cellulase,better substrate affinity,wider pH adaptability,better thermostability and tolerance to ethyl alcohol. The optimum pH value was 6. 0,and the optimum temperature was 40℃. With SC-5,simultaneous saccharification and fermentation of 1. 0 kg of sweet sorghum stalks could produce 45 g of ethanol. All these indicated that the fermentation process was simpler and the production efficiency was higher.

关 键 词：低温纤维素酶 甜高粱秆 同步发酵 纤维素乙醇 

分 类 号：S514.099[农业科学—作物学]