棉花种子老化过程中生理生化指标变化比较研究  

Comparative Study on Physiological and Biochemical Indexes Changes in the Aging Process of Cotton Seeds

作　　者：徐敏 李憬霖 朱鹤 金路路 王子胜 

机构地区：[1]辽宁省经济作物研究所,辽宁辽阳111000 [2]沈阳农业大学农学院,沈阳110161

出　　处：《种子》2018年第2期14-18,共5页Seed

基　　金：国家七大作物育种专项<棉花强优势杂交种的选育及示范推广>(2016YFD 0101400);国家棉花产业技术体系辽河综合试验站(2016-2020;CARS-18-31);国家棉花转基因重大专项<北方特早熟棉区早熟抗病转基因棉花新品种培育>(2016-2020;2016ZX 08005-002)

摘　　要：为探索棉花种子贮藏过程对种子活力的影响,选取辽棉23号、辽棉31两个品种的棉花种子,以毛籽及光籽为试验材料,在T=40℃、R=100%的条件下分别进行老化处理2,4,6d和老化处理8d。将种子激活后,测定种子渗出液电导率、丙二醛(MDA)含量、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性。结果表明,随着老化时间的延长,渗出液电导率和丙二醛(MDA)含量升高,酶(POD、CAT、SOD)活性下降,并且不同品种、不同状态(光籽和毛籽)之间表现不同。结合各处理间发芽情况,认为棉花种子贮藏过程中,应根据不同的情况区别对待以保证其活性,生产中也可以利用热击处理(温汤浸种)以提高种子的发芽率。In order to explore the impact of storage on cotton seed vigor, smooth seed and hair seed o{ two cotton cultivars（Liaomian 23 ＆Liaomian 31）were selected as experimental materials, which trea- ted under the condition of T=40℃ .R= 100% ,aging 2 days.4 days.6 days and 8 days.After seed acti- vation, determined exudate conductivity. MDA content. POD. CAT. SOD activity. The conclusion showed that： as the aging time increased, conductivity and MDA content increased, enzyme（POD, CAT,SOD） activity decreased, and performance was different between different cultivars and seed states （smooth seeds and hair seed）. In combination with the germination situation between the groups, thinking of cotton seed storage process, It should be treated differently according to different situations in order to assure its activity,also can use in the production of heat shock treatment（luke- warm water soaking seed）in order to improve the germination rate of seeds.

关 键 词：棉花种子 老化 渗出液电导率 丙二醛(MDA) 过氧化物酶(POD) 过氧化氢酶(CAT) 超氧化物歧化酶(SOD) 

分 类 号：S562[农业科学—作物学]