基于香豆素衍生物的反应型硫化氢荧光探针的合成与应用  

Synthesis and application of reactive hydrogen sulfide fluorescent probe based on coumarin derivarives

作　　者：侯鹏[1] 董玉晶[1] 刘磊[1] 夏春辉[1] 李爽[1] 

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《中国测试》2018年第2期56-61,共6页China Measurement & Test

基　　金：2016年度黑龙江省教育厅基本科研业务费项目(科技类)(2016-KYYWF-0855)

摘　　要：该文基于光诱导电子转移(PET)机制,利用H_2S诱导探针分子结构中的2,4-二硝基苯醚水解,抑制PET过程,合成一种反应型H_2S荧光探针。利用NMR和MS对探针1的结构进行表征。通过光谱学测试和生物学细胞实验证实,相对于其他测试物质(阴、阳离子及生物分子),它对H_2S具有良好的选择性和灵敏度,可以在生理条件下检测H_2S。当H_2S加入到探针溶液后,溶液呈现出绿色荧光,在490 nm荧光强度恢复75倍,当探针的浓度为10.0μmol/L时,其对H_2S的检测限为3.0×10^(-8) mol/L;同时,探针成功实现细胞内H_2S的荧光成像,为其在生物学及医学中的实际应用奠定实验基础。In this paper, based on the photoinduced electron transfer(PET) mechanism, a reactive H_2S fluorescent probe was successfully synthesized by H_2S induced the hydrolysis of 2, 4-dinitrophenyl ether to inhibit PET process. The structure of probe 1 was characterized by NMR and MS.Compared with other test material(anion, ion and biological molecules), it has good selectivity and sensitivity for H_2S probe to detect H_2S under physiological conditions. With the addition of H_2S,the solution showed green fluorescence and the fluorescence intensity restore 75-fold at 490 nm.When the concentration of the probe is 10.0 μmol/L, the detection limit of H_2S is 3.0×10^(-8) mol/L.At the same time, the probe of H_2S in cell fluorescence imaging showed that it would play a experimental foundation for the practical application in biology and medicine.

关 键 词：硫化氢 香豆素 荧光检测 探针 

分 类 号：O657.3[理学—分析化学]