选择性肠道去污染对创伤性截瘫家兔小肠组织MDA含量和SOD、GSH-Px的影响  

Effect of selective decontamination of the digestive tract on MDA concentration and SOD,GSH-Px activities of small intestinal tissue in rabbits with complete paraplegia

作　　者：聂海 刘鹏 曹德乾 白春宏[2] 周际 刘齐东 安洪[3] 

机构地区：[1]四川省医学科学院.四川省人民医院东区骨科,成都610101 [2]武警后勤学院附属医院脊柱一科,天津300163 [3]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《创伤外科杂志》2018年第2期121-125,共5页Journal of Traumatic Surgery

基　　金：四川省卫生厅科研课题(120579)

摘　　要：目的探讨选择性肠道去污染(selective decontamination of the digestive tract,SDD)对创伤性截瘫家兔肠道组织丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧物酶(glutathione peroxidase,GSH-Px)的影响,明确SDD对截瘫家兔肠道氧化应激损伤的保护作用。方法建立截瘫家兔模型。选择40只体质量为2.0~2.5kg 6月龄清洁级健康家兔,雌雄不限,随机分为处理组和对照组,每组20只;另外增加正常对照组家兔10只。采用PTA方案[多粘菌素E(P)、妥布霉素(T)和两性霉素B(A)]进行SDD处理4d。4d后处死家兔,采集距回盲部5cm左右小肠标本进行HE染色观察小肠黏膜变化,并检测小肠组织MDA含量和SOD、GSH-Px水平。结果 SDD处理组小肠组织MDA水平较对照组明显降低[(3.58±0.98)nmo L/mgprot vs.(8.19±1.22)nmo L/mgprot,P<0.001];处理组SOD活性明显高于对照组[(57.2±9.4)U/mgport vs.(42.7±13.4)U/mgport,P<0.001];处理组GSH-Px活性也明显高于对照组[(111.6±12.0)U/mgport vs.(89.7±11.0)U/mgport,P<0.001]。相关分析表明,小肠HE染色Chiu病理评分与小肠组织MDA含量呈显著正相关(P<0.001,r=0.649),与SOD(r=-0.387)、GSHPx(r=-0.531)活性呈显著负相关(P<0.001)。结论 SDD处理截瘫家兔后可减弱肠道脂质过氧化反应,减轻氧化应激损伤,从而保护肠黏膜。Objective To explore effects of selective digestive decontamination（SDD） on the content of malondialdehyde（MDA）,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）,and its effects on oxidative stress reaction in small intestinal mucosa in paraplegic rabbits. Methods Forty rabbits were subjected to a complete paraplegia following spinal cord injury,and divided into SDD-treated（n = 20） and control groups（n = 20）.The treatment group received SDD using PTA（polymyxin,tobramycin and amphotericin B） protocol by gavage three times daily for 4 d after injury. The control group was given the same amount of normal saline. Samples from the small intestine were collected to detect MDA content,SOD and GSH-Px activities and to assess morphological changes in the small intestine,using Hematoxylin and eosin staining. The correlation between MDA,SOD and GSH-Px levels and the degree of intestinal mucosal damage were analysed. Results The content of MDA[（3. 58 ± 0. 98）nmol/mgprot vs.（8. 19 ± 1. 22） nmo L/mgprot,P 〈0. 001] in intestinal tissue was significantly lower in the SDD group than those in the control group（P〈 0. 001）. The activities of SOD [（57. 2 ± 9. 4） U/mgport vs.（42. 7 ±13. 4） U/mgport] and GSH-Px[（111. 6 ± 12. 0） U/mgport vs.（89. 7 ± 11. 0） U/mgport] were obviously higher in the SDD group than those in the control group. Correlation alalysis showed a positive correlation between MDA content and degree of intestinal mucosal damage（P〈 0. 001,r = 0. 649）,and a negative relation between SOD（r =-0. 387）,GSH-Px（r =-0. 531） activities and degree of intestinal mucosal injury（P 〈0. 001）. Conclusion SDD protects against lipid peroxidation in intestinal tissues and reduces oxidative stress damage in rabbits with complete paraplegia.

关 键 词：创伤性截瘫 小肠 选择性肠道去污染 氧化应激反应 兔 

分 类 号：R641[医药卫生—外科学] R651.2[医药卫生—临床医学]