表达猪圆环病毒2型Cap蛋白的重组猪瘟兔化弱毒疫苗株的构建与鉴定  

Generation and characterization of recombinant classical swine fever virus C-strain expressing the cap protein of porcine circovirus type 2

作　　者：张玲楷 李永锋[1] 谢利豹 孙元[1] 王晓[1] 仇华吉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150069

出　　处：《生物工程学报》2018年第2期216-223,共8页Chinese Journal of Biotechnology

基　　金：哈尔滨市优秀学科带头人项目(No.2014RFXYJ045)资助~~

摘　　要：猪瘟(CSF)是由猪瘟病毒(CSFV)引起的一种毁灭性传染病,给养猪业造成重大经济损失。猪瘟兔化弱毒疫苗(C株)是一株非常安全、有效的优秀弱毒疫苗,对各年龄和品种的猪都极其安全,同时对不同基因亚型的CSFV均能提供有效的免疫保护。在现地,CSFV和猪圆环病毒2型(PCV2)混合感染的现象时常发生,有必要研制针对这两种病毒混合感染的二价疫苗。本研究首次构建了表达PCV2 Cap蛋白的重组C株,并评价了其在体内外的特性。结果表明,该重组病毒与C株具有相近的体外增殖特征,能够稳定表达Cap蛋白,在家兔体内具有与C株相似的生物学表型,在免疫家兔后10 d,抗CSFV E2抗体全部转阳,然而抗Cap抗体未能转阳。本研究为进一步优化表达PCV2Cap蛋白的重组C株奠定了基础。Classical swine fever（CSF）, caused by classical swine fever virus（CSFV）, is a devastating viral disease in swine, leading to significant economic losses to the pig husbandry. C-strain is one of the best modified live vaccines against CSF. The vaccine is highly safe and efficacious and can provide rapid and complete protection against essentially all genotypes of CSFV. Co-infections of pigs with CSFV and porcine circovirus type 2（PCV2） occur frequently in the field, making it difficult to control the associated diseases. Here, a recombinant C-strain r HCLV-Cap expressing the Cap protein of PCV2 was constructed and evaluated in vitro and in vivo. The recombinant had comparable phenotypes to C-strain in cell cultures and rabbits. At ten days post-immunization, anti-E2, but not anti-Cap, antibodies were detected in the rabbits inoculated with the recombinant virus. Our study warrants further work to construct C-strain-based bivalent vaccines.

关 键 词：猪瘟病毒 猪圆环病毒2型 活病毒载体 免疫原性 

分 类 号：S852.65[农业科学—基础兽医学]