UV+ARTP复合诱变筛选子囊霉素高产菌株  

Screening of High-yield Ascomycin-producing Strain by UV Combined with ARTP Mutation

作　　者：任林英[1] 张祝兰[1] 王德森[1,2] 杨煌建 连云阳[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007 [2]福建科瑞药业有限公司,福建福州350007

出　　处：《中国医药工业杂志》2018年第2期181-185,共5页Chinese Journal of Pharmaceuticals

基　　金：福建省属公益类研究所基本科研专项(2015R1009-8);福建省化药技术重大研发平台(2014Y2001);福州市科技计划项目(2016-G-69);福清市科技计划项目(融科2016(21))

摘　　要：采用紫外(UV)与常压室温等离子体(ARTP)复合诱变技术对子囊霉素产生菌吸水链霉菌Streptomyces hygroscopicus进行诱变处理,结合摇瓶发酵初筛和复筛,以HPLC法检测子囊霉素的含量,结果获得1株遗传性状良好的子囊霉素高产菌株S.hygroscopicus FIM-38-24,其产子囊霉素能力较出发菌株提高4倍以上,发酵单位达568.0μg/ml。The spore suspension of ascomycin producing strain Streptomyces hygroscopicus was mutated by ultraviolet （UV） and atmospheric and room temperature plasma （ARTP）, and a high-yield mutant strain Streptomyces hygroscopicus FIM-38-24 was obtained through primary screening and rescreening by flask shaking fermentation. The content of ascomycin was determined by HPLC. The results showed that the ascomycin production capacity of the mutant FIM-38-24 （568.0 μg/ml） was 4 times of that of the original strain.

关 键 词：子囊霉素 吸水链霉菌 常压室温等离子体 复合诱变 

分 类 号：TQ465.92[化学工程—制药化工]