谱系示踪技术揭示Tbx18^+肾祖细胞分化为脂肪细胞的潜能  

Genetic Fate-mapping Reveals the Differentiation Potential of Tbx18^+ Renal Progenitor Cells into Adipocytes

作　　者：袁欣[1] 佘强[2] 杜建霖[2] 

机构地区：[1]重庆医科大学附属第二医院肾内科 [2]重庆医科大学附属第二医院心内科

出　　处：《中国生物化学与分子生物学报》2018年第2期177-182,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.81770251);重庆教委科学技术研究项目(No.KJ130324);重庆市科委前沿与应用基础研究计划项目(No.cstc2015jcyj A10070)资助~~

摘　　要：转录因子Tbx18在泌尿系发育中发挥重要作用。利用遗传谱系示踪模型,揭示了Tbx18^+肾祖细胞具有向多种肾系细胞分化的潜能。但尚无文献报道其是否具有向脂肪细胞分化的潜能。本研究通过对Tbx18Cre/Rosa26^(LacZ)双杂合小鼠泌尿系组织进行整体X-gal染色发现,肾包膜、输尿管及肾周脂肪组织能特异性表达β-gal蛋白,说明肾包膜、输尿管及肾周脂肪组织可能来源于Tbx18+祖细胞。对Tbx18Cre/Rosa26^(EYFP)双杂合小鼠泌尿系组织进行免疫荧光染色,发现部分脂滴相关蛋白^+(perilipin)脂肪细胞能表达标记蛋白EYFP,说明部分泌尿系脂肪细胞来源于Tbx18^+祖细胞。本研究揭示了Tbx18^+祖细胞具有分化为脂肪细胞的潜能,进一步证实了Tbx18^+肾祖细胞的多分化潜能。结合本研究结果,若进一步研究肾损伤时,来源于Tbx18^+祖细胞的泌尿系脂肪细胞是否进一步增多,将会为肾损伤的再生修复提供一些思路和启发。The T-box transcription factor 18（ Tbx18） plays an essential role in normal development of the urinary system. It has been identified that Tbx18-positive cells contribute to multiple components of the mature urogenital system. However,it remains unclear whether Tbx18-positive progenitors play a specific role in the development of urologic fat tissues. Here we applied the Tbx18 lineage tracing model of Tbx18 Cre/Rosa26^EYFP and Tbx18 Cre/Rosa26^LacZ mice to identify the differentiation capacity of Tbx18-expressing progenitor cells in the urologic system. X-gal staining of the urinary tissue revealed that the Xgal protein is detected in the renal capsule,the ureter and perirenal fat tissue and that Tbx18-expressing precursors contribute to the renal capsule,ureter and perirenal adipocytes in Tbx18 Cre/Rosa26^LacZ fatemapping mice. Immunostaining of the kidney and perirenal tissue revealed some yellow fluorescent protein-positive（ YFP^＋） cells express Perilipin,the marker for adipocytes and that Tbx18-positive progenitors contribute to perirenal adipocytes in Tbx18 Cre/Rosa26^EYFP fate-mapping mice. Thus,our analyses identified the multi-differentiation capacity of Tbx18-expressing progenitors in the urinarysystem. Further researches are needed to study the capacity of Tbx18-expressing progenitors to differentiate into adipocytes and other components of the urogenital system in models of kidney injury.

关 键 词：T-box转录因子18 脂肪细胞 祖细胞 谱系示踪 

分 类 号：R692[医药卫生—泌尿科学] R394.2[医药卫生—外科学]