体外G-CSF刺激对健康人外周血淋巴细胞亚群分布的影响  

Effect of G-CSF in vitro Stimulation on Distribution of Peripheral Lymphocyte Subsets in the Healthy Persons

作　　者：赵莎莎[1,2] 方姝[1] 朱成英 王莉莉[1] 高春记 

机构地区：[1]解放军总医院血液科,北京100853 [2]南开大学医学院,天津300071

出　　处：《中国实验血液学杂志》2018年第1期258-262,共5页Journal of Experimental Hematology

基　　金：北京市自然科学基金(7162175)

摘　　要：目的:探讨体外粒细胞集落刺激因子(G-CSF)对健康人外周血淋巴细胞亚群分布的影响。方法:采集8例健康人的外周血,密度梯度离心法分离单个核细胞(MNC),并以200 ng/ml G-CSF、200 ng/ml G-CSF^+10μg/ml ConA体外培养3 d,流式细胞术检测不同培养条件下健康人外周血淋巴细胞亚群的分布。结果:与对照组相比,GCSF和G-CSF^+Con A组T淋巴细胞水平显著增高(P<0.01,P<0.05),而3组间B淋巴细胞、NK细胞水平表达无明显差异;进一步分析G-CSF对健康人外周血T淋巴细胞亚群分布影响的结果发现,与对照组相比,G-CSF组CD4^+T、CD8^+T淋巴细胞水平均明显增高(P<0.01,P<0.05),Treg细胞水平无明显变化;与对照组相比,G-CSF^+ConA组CD4^+T、CD8^+T、Treg细胞水平均显著增高(P<0.05,P<0.01,P<0.01);T淋巴细胞表面G-CSF受体分析发现,与对照组相比,G-CSF^+ConA组T淋巴细胞表面G-CSF受体表达显著升高(P<0.01)。结论:体外G-CSF刺激能增加健康人外周血CD4^+T、CD8^+T淋巴细胞的水平;Con A可提高T淋巴细胞的水平,诱导其表面G-CSF受体表达。Objective： To investigate the effect of granulocyte-colony stimulating factor（ G-CSF） in vitro stimulation on the distribution of lymphocyte subset in healthy human. Methods： Peripheral blood mononuclear cells（ PBMNCs） were collected from 8 healthy volunteers by density gradient centrifugation on Ficoll-Paque^TM. In vitro 200 ng/ml G-CSF or 200 ng/ml G-CSF plus 10 μg/ml ConA directly act on PBMNCs,then the colleted cells were cultivated for 3 days. Lymphocyte subsets were stained with the corresponding fluoresce labeled antibodies and detected by flowcytometry. Results： The levels of T cells in G-CSF group and G-CSF ＋ ConA group were both higher than that in the control group（ P〈0. 001,P〈0. 05）. However,there were not significantly different in B cells and NK cells levels among the 3 groups. Furthermore,analysis of the effect of G-CSF on T cell subsets indicated that the levels of CD4^＋T cells and CD8^＋T cells in G-CSF group were both significantly higher than those in control group（ P〈0. 01,P〈0. 05）,Treg cells was not different between G-CSF and control group. Compared with the control group,the level of CD4^＋T cells,CD8^＋T cells and Treg cells in G-CSF ＋ ConA group significantly increased（ P〈0. 05,P〈0. 01,P〈0. 01）. Analysis of G-CSF receptor（ G-CSFR） expression showed that G-CSFR expression on T cells in G-CSF ＋ ConA group dramatically increased,as compared with control group（ P〈0. 01）. Conclusion： The levels of CD4^＋T cells and CD8^＋T cells in healthy human peripheral blood can be increased by G-CSF stimulation. ConA can enhance the level of T cells and induce G-CSFR expression on T cells.

关 键 词：粒细胞集落刺激因子 刀豆蛋白 淋巴细胞亚群 

分 类 号：R973.4[医药卫生—药品]