蛋白聚糖-Aggrecan G1区诱导小鼠脊柱炎和骶髂关节炎模型的建立  

Induction of Spondylitis and Sacroiliitis in Mice by the G_1 Domain of the Proteoglycan Aggrecan

作　　者：韩青 梁强 吴振彪[1,2] 朱平[1,2] 

机构地区：[1]中国人民解放军第四军医大学西京医院临床免疫科,西安710032 [2]中国人民解放军第四军医大学国家分子医学转化科学中心-临床分子医学研究所 [3]中国人民解放军第四军医大学细胞工程中心

出　　处：《医学研究杂志》2018年第1期20-22,共3页Journal of Medical Research

基　　金：国家重点基础研究发展计划“973”计划项目(2015CB553704)

摘　　要：目的建立强直性脊柱炎BALB/c小鼠实验动物模型,探讨人Aggrecan G_1区纯化蛋白在脊柱炎和骶髂关节炎发病中的作用。方法人Aggrecan G_1纯化蛋白和弗氏佐剂免疫BALB/c小鼠,诱导产生脊柱炎和骶髂关节炎,建立强直性脊柱炎实验动物模型。采用Micro-CT对小鼠骨骼进行3D重建和组织病理学方法鉴定脊柱炎小鼠的发病程度和病理学特征。结果用人Aggrecan G_1区纯化蛋白和弗氏完全佐剂免疫后,实验小鼠脊柱炎和骶髂关节炎的发生率分别为46%和9%。组织病理学显示,椎间盘间隙变窄,局部大量软骨细胞聚集,骨赘形成。micro-CT 3D重建显示,椎间盘间隙变窄,骶髂关节面骨侵蚀。结论成功建立和鉴定了人Aggrecan G_1区纯化蛋白诱导的小鼠脊柱炎实验动物模型,为深入研究强直性脊柱炎的发病机制奠定实验基础。Objective To set up ankylosing spondylitis BALB/c mice experimental animal model and discuss the Aggrecan G1 purified protein in the role of spondylitis and sacroiliitis. Methods Human - purified Aggrecan G1 domain and FCA were injected into BALB/c mice to induce spondylitis and sacroiliitis. Spondylitis and sacro iliitis were determined by pathological examination and radiology. Results It was shown that immunity to human - purified Aggrecan G1 domain resulted in spondylitis in the lumbar spine and sacroiliitis in 46% and 9% mice respectively. Accumulation of chondrocytes was observed in spinal intervertebral disc and the rodent sacroiliac joints. Conclusion We successfully established and identified Aggrecan G1 area with purified protein induced spondylitis experimental animal model of mice,which will help to probe the further research on the pathogenesis ankylosing spondylitis.

关 键 词：强直脊柱炎 动物模型 蛋白聚糖 AGGRECAN 

分 类 号：R3[医药卫生—基础医学]