姜黄素对阿尔茨海默病模型大鼠海马CA1区细胞凋亡干预的研究  

Effect of Curcumin on Neuron Apoptosis in the Hippocampus Area CA1 of Alzheimer's Disease Model Rats

作　　者：叶茂盛[1] 潘锋[1] 周勇[1] 蒋蓝英[1] 傅仲颖 赵东杰[1] 

机构地区：[1]浙江省中西医结合医院老年病科,杭州310003

出　　处：《医学研究杂志》2018年第2期92-95,106,共5页Journal of Medical Research

基　　金：浙江省中医药科技计划项目(2013ZB097,2015ZB083);杭州市科技发展计划项目(20150733Q40)

摘　　要：目的探讨姜黄素对阿尔茨海默病(AD)模型大鼠学习记忆能力及海马CA1区细胞凋亡的影响。方法将45只SD雄性大鼠分为3组,既假手术组(NS组)、Aβ模型组(AB组)和姜黄素干预组(CU组)。AB组和CU组直接注射Aβ1-42于大鼠海马CA1区;CU组用姜黄素给予大鼠连续灌胃30天。采用Morris水迷宫测试大鼠学习记忆能力,采用TUNEL法检测海马CA1区神经细胞凋亡。结果姜黄素可以缩短AD模型大鼠水迷宫测试的潜伏时间,改善大鼠学习记忆能力。Aβ模型组海马组织细胞出现明显的细胞凋亡,姜黄素干预组海马TUNEL阳性神经细胞数目(24.63±3.28个)比Aβ模型组(45.92±2.71个)明显减少(P<0.01)。结论姜黄素可明显改善Aβ所致AD大鼠学习记忆功能,姜黄素可显著改善大鼠海马CA1区Aβ诱导的细胞凋亡。Objective To investigate the effect of curcumin on the ability of learning and memory, neuron apoptosis in the hippo- campus area CA1 of Alzheimer＇s disease model rats. Methods Forty five Sprague - Dawley rats were assigned into 3 groups. Rats in the sham -operated group（NS） were injected with normal saline. Rats in the Aβ group（AB） and curcumin group （RG） were injected with Aβ1-42 Rats in the curcumin group were feeded with curcumin solution for 30 days consecutively. The Morris water maze was used to in- vestigate the ability of learning and memory in the rats. The apoptotic neurons in hippocampus area CAI were detected by TUNEL method. Results Curcumin can shorten the incubation period of experimental rats testing in water maze. The number of TUNEL positive neuron in hippoeampus area CA1 of cureumin group（24.63 ±3.28）is obviously lower than the Aβ group（45.92 ± 2.71 ）, the difference between the two groups is statistically significant （ P 〈 O. O1 ）. Conclusion Curcumin can ameliorate the learning and memory ability of AD rats. Curcumin can reduce the Aβ -induced apoptosis in the hippocampus area CA1 of Alzheimer＇s disease model rats.

关 键 词：阿尔茨海默病 姜黄素 Β-淀粉样蛋白 细胞凋亡 

分 类 号：R3[医药卫生—基础医学]