杉木种鳞石蜡切片工艺优化研究  

Process Optimization of Paraffin Section Method for Scale of Cunninghamia lanceolata

作　　者：左丹丹[1,2] 熊升 李佳 饶丽莎 林思祖[1,2] 陈宇[1,2] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]国家林业局杉木工程技术研究中心,福建福州350002

出　　处：《西南林业大学学报（自然科学）》2018年第1期59-65,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家林业局杉木工程技术研究中心平台建设项目(ptjh130002)资助;福建农林大学校重点项目建设专项(6112c0310)资助;林学学科建设项目(6112c0300)资助

摘　　要：为精确观察杉木种鳞内部的细胞结构,经过对2种不同固定液、3种不同软化处理、4种不同脱水与透明处理、优化浸蜡时间和温度以及后续包埋、染色等工艺步骤对比,优化了杉木种鳞石蜡切片工艺。结果表明:杉木种鳞石蜡切片的优化工艺和方法为,FAA和FPA两种固定液固定效果相同,均能有效的固定材料;12 h的盐酸水溶液软化效果最佳;最佳脱水时间为每级2 h,透明时间为每级1 h。该优化工艺有效克服了蜡带不连续、切片不完整的缺陷,可获得清晰完整的杉木种鳞细胞结构图。In order to accurately observe the ceil internal structure of Cunninghamia lanceolata scales, the processes, which was summarized by comparing the results of different treatments including 2 different fixing solu- tions, 3 softening treatments, 4 dehydrant and transparent treatments, time and temperature of waxing, embedding and dyeing, were optimized. Results showed that the material could be both fixed effectively by the FAA and FPA solutions ; the best softening process was the hydrochloric acid solution for 12 h ; the best dehydration time was 2 h per level, and the best transparent time was 1 h per level. Clear and complete cell structure map of the scales of C. lanceolata could be obtained by using this method.

关 键 词：杉木 种鳞 石蜡切片 工艺优化 

分 类 号：S791.27[农业科学—林木遗传育种]