黄芪党参对心肌梗死后心衰小鼠心肌复极时间的干预研究  

Effect of Astragalus membranaeus and Codonopsis pilosula on Myocardial Repolarization in Post-infarction Heart Failure Mice

作　　者：李思耐[1,2] 林谦 高群[1] 武乾 崔海峰[3] 刘静[1] 石晓路[3] 

机构地区：[1]北京中医药大学东方医院心血管内科,北京100078 [2]首都医科大学附属北京中医医院北京市中医研究所,北京100010 [3]中国中医科学院医学实验中心,北京100700

出　　处：《中国中西医结合杂志》2018年第2期232-236,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173176;No.81373779)

摘　　要：目的研究益气药黄芪、党参对心肌梗死(myocardial infarction,MI)后心衰小鼠心肌细胞复极时间的干预作用。方法通过冠脉结扎术复制MI后心衰小鼠模型,采用随机数字表分为假手术组(生理盐水)、模型组(生理盐水)、益气组[黄芪1.2g/(kg·d)+党参0.9g/(kg·d)配方颗粒溶液]、西药组[美托洛尔溶液8mg/(kg·d)]。术后灌胃干预4周。检测小鼠超声心动及心电图,分离心肌细胞,使用全细胞膜片钳技术记录分析心肌细胞动作电位。结果黄芪、党参可改善MI后心衰小鼠左室射血分数(P<0.05),缩短MI后心衰小鼠QT间期(P<0.01)及校正QT间期(P<0.05),减少室性心律失常发生率,缩短MI后心衰小鼠心肌细胞复极时间(P<0.05),并且缩短心肌细胞复极时间效果优于美托洛尔(P<0.05)。结论益气药黄芪、党参可提高MI后心衰模型心肌复极速度,减少室性心律失常发生率,改善心功能和远期预后。Objective To explore the effect of qi-promoting drugs （Astragalus membranaeus and Codonopsis pilosula ） on myocardial repolarization in post-infarction heart failure mice. Methods The post-infarction heart failure animal models were established by the ligation of left anterior descending coro- nary in mice. The mice were divided into the sham group （administered with normal saline by gastrogavage）, control group （administered with normal saline by gastrogavage）, qi-promoting group E administered with As- tragalus membranaeus （1.2 g · kg -1· d -1 ） and Codonopsis pilosula （0. 9 g ·kg-1·d-1 ） granular solution by gastrogavage ~ and Metoprolol group （administered with Metoprolol 8 mg · kg-1 . d-1 by gastrogavage） by ran- dom digit table. After 4 weeks of intervention, echocardiography and electrocardiogram were examined. Then the cardiomyocytes of mice were isolated and the action potentials of cardiomyocytes were recorded by patch clamp. Results The left ventricular ejection fractions of post-infraction heart failure mice were improved by qi- promoting drugs （P 〈0.05）. After treatment with qi-promoting drugs, the QT intervals （P 〈0.01 ） and the cor- rected QT intervals （P 〈0. 05） were shortened and the risk of ventricular arrhythmia was reduced, the repolar- ization time of cardiomyocytes were shortened （P 〈0.05）, and the effect of shortening cardiomyocytes rep- darization time was better than Metoprolol （P 〈0.05）. Conclusion These findings revealed that treatment with qi-promoting drugs Astragalus membranaeus and Codonopsis pilosula accelerated the myocardial repolariza- tion, reduced the risk of ventricular arrhythmia and improved cardiac function and prognosis in post-infarction heart failure.

关 键 词：心律失常 心力衰竭 心肌梗死 黄芪 党参 

分 类 号：R285.5[医药卫生—中药学]