右美托咪定对梗阻性黄疸大鼠肠黏膜损伤的影响  

Effect of dexmedetomidine on damage to intestinal mucous membrane of rats with obstructive jaun-dice

作　　者：李晓燕[1] 解雅英[1] 于建设[1] 石海霞[1] 孙俊枝[1] 

机构地区：[1]内蒙古医科大学第一附属医院麻醉科,呼和浩特市010010

出　　处：《中华麻醉学杂志》2017年第11期1311-1313,共3页Chinese Journal of Anesthesiology

基　　金：内蒙古医科大学附属医院重大基金（NYFYZD2014016）;内蒙古科技厅科技自然资金[2015MS（LH）0809]

摘　　要：目的评价右美托咪定对梗阻性黄疸大鼠肠黏膜损伤的影响。 方法清洁级健康雄性SD大鼠30只，4～6月龄，体重200～250 g，采用随机数字表法分为3组（n＝10）：对照组（C组）、梗阻性黄疸组（OJ组）和右美托咪定组（D组）。采用双重结扎胆总管的方法制备大鼠梗阻性黄疸模型。D组于造模后3 d腹腔注射右美托咪定负荷量100 μg/kg，然后以50 μg·kg－1·h－1的速率腹腔输注5 h，C组和OJ组给予等容量生理盐水。右美托咪定给药后5 h时采集心脏血样，采用ELISA法测定血清二胺氧化酶（DAO）和TNF-α浓度。随后处死大鼠取结肠组织，观察病理学结果。 结果与C组比较，OJ组和D组血清DAO和TNF-α浓度升高（P〈0.05）；与OJ组比较，D组血清DAO和TNF-α浓度下降（P〈0.05）。D组肠组织病理学损伤较OJ组减轻。 结论右美托咪定可减轻梗阻性黄疸大鼠肠黏膜损伤。Objective To evaluate the effect of dexmedetomidine on the damage to intestinal mu- cous membrane of rats with obstructive jaundice. Methods Thirty pathogen-free healthy male Sprague- Dawley rats, aged 4-6 months, weighing 200-250 g, were divided into 3 groups （n= 10 each） using a random number table： control group （group C）, obstructive jaundice group （group O J） and dexmedeto- midine group （ group D）. Obstructive jaundice was induced by double ligation of common bile duct in anes- thetized rats. In group D, dexmedetomidine was intraperitoneally injected in a loading dose of 100 μg/kg at 3 days after establishment of the model, followed by intraperitoneal infusion of 50 μg·kg-1·h-1 for 5 h. The equal volume of normal saline was given instead in C and OJ groups. At 5 h after administration of dexmedetomidine, blood samples were collected from the heart for determination of serum concentrations of diamine oxidase （DAO） and tumor necrosis factor-alpha （TNF-a） by enzyme-linked immunosorbent as- say. Then the rats were sacrificed and colon tissues were removed for microscopic examination of the patho- logical changes. Results Compared with group C, the serum DAO and TNF-a concentrations were signifi- cantly increased in OJ and D groups （P〈0.05）. Compared with group OJ, the serum DAO and TNF-a concentrations were significantly decreased in group D （P〈0.05）. The pathological changes were signifi- cantly attenuated in group D when compared with group OJ. Conclusion Dexmedetomidine can reduce the damage to intestinal mucous membrane of rats with obstructive jaundice.

关 键 词：右美托咪啶 黄疸 阻塞性 肠黏膜 

分 类 号：R614[医药卫生—麻醉学]