UPLC-MS/MS测定大鼠血浆中26-OH-人参二醇浓度的不确定度评定  

Uncertainty in concentration determination of 26-OH-PD in human plasma by UPLC-MS/MS

作　　者：林美妤 张楠淇 王翠竹[2] 朱海林[2] 李平亚[2] 刘金平[2] 

机构地区：[1]湖南中医药大学,长沙410208 [2]吉林大学再生医学科学研究所新药研究室,长春130021

出　　处：《中南药学》2018年第2期155-160,共6页Central South Pharmacy

摘　　要：目的评定超高效液相色谱-串联质谱(UPLC-MS/MS)法测定大鼠血浆中26-OH-人参二醇浓度的不确定度。方法分析UPLC-MS/MS法测定大鼠血浆中26-OH-人参二醇浓度的不确定度来源,根据各分量计算出合成不确定度并进行了扩展。结果大鼠血浆中低(13.87 ng·mL^(-1))、中(188.04ng·mL^(-1))和高(824.33 ng·mL^(-1))浓度26-OH-人参二醇的扩展不确定度分别为3.63、12.41和66.61ng·mL^(-1)(P=95%,k=2)。结论 UPLC-MS/MS法测定大鼠血浆中26-OH-人参二醇低浓度样品的不确定度主要分别由线性拟合引入,中、高浓度样品的不确定度主要由基质效应引入。该法适用于评定UPLC-MS/MS法测定血浆中26-OH-人参二醇的不确定度研究,能为复杂生物样品分析过程的不确定度评定提供一定参考。Objective To evaluate the uncertainty in 26-OH-PD determination in human plasma by UPLC- MS/MS. Methods The whole process of 26-OH-PD determination was analyzed and the uncertainty from various sources was calculated. The combined and expanded uncertainty was also evaluated. Results The expanded uncertainty of 26-OH-PD at low （13.87 ng·mL^-1）, medium （188.04 ng·mL^-1） and high （824.33 ng·mL^-1） level was 3.63, 12.41 and 66.61 ng·mL^-1 （P=95%, k = 2）. Conclusion Calibration curve fitting is the main source of uncertainty for the low concentration of 26-OH-PD, and matrix effect is the main source of uncertainty for the 26-OH-PD determination at high and medium concentration. This method can be used to evaluate the uncertainty of 26-OH-PD determination in the plasma. Reference is also provided for the uncertainty evaluation in drug analysis.

关 键 词：不确定度 26-OH-人参二醇 UPLC-MS/MS 血药浓度 

分 类 号：R917[医药卫生—药物分析学]