3种不同组织来源的间充质干细胞促内皮祖细胞血管形成作用的比较  

Angiogenic ability of 3 different tissues-derived mesenchymal stem cells on endothelial progenitor cells

作　　者：王斯琪 卢海源 程腊梅 

机构地区：[1]中南大学基础医学院生殖与干细胞工程研究所,长沙410078

出　　处：《中南大学学报（医学版）》2018年第2期184-191,共8页Journal of Central South University ：Medical Science

基　　金：国家高技术研究发展计划(863计划)(2011AA020113)~~

摘　　要：目的:比较人骨髓、脂肪和脐带来源的间充质干细胞(mesenchymal stem cells,MSCs)促进内皮祖细胞(endothelial progenitor cells,EPCs)形成血管以及维持血管稳定的能力。方法:采用体外共培养成血管试验比较3种来源的MSCs促EPCs形成管状结构的能力;采用体内基质胶Matrigel成血管试验、免疫组织化学染色比较3种MSCs促进EPCs在体内形成功能血管的能力。结果:EPCs在脂肪MSCs单层上形成的管状结构长度和节点数均高于在骨髓和脐带MSCs单层上的形成数;EPCs与脂肪MSCs在Matrigel上共培养时形成的毛细血管样结构稳定性高于骨髓和脐带MSCs;脂肪MSCs与EPCs在体内Matrigel中形成大量有血流灌注的功能血管,脐带MSCs与EPCs在体内Matrigel中形成少量有血流灌注的功能血管,而骨髓MSCs与EPCs在体内Matrigel中只形成有血细胞渗漏的不完整血管。结论:脂肪MSCs在体内、体外促EPCs形成血管结构并维持其稳定的能力高于骨髓和脐带MSCs。Objective： To compare the ability between bone marrow-derived mesenchymal stem cells （MSCs） （BM-MSCs） and adipose-derived MSCs （AD-MSCs） or umbilical cord-derived MSCs （UC-MSCs） on promotion of vessels formation and vessels stabilization relevant to the functions of EPCs. Methods： In vitro, co-culture blood vessel test was performed to compare the angiogenic ability between BM-MSCs, AD-MSCs or UC-MSCs. In vivo, angiogenic assay dependent on basement membrane matrix Matrigel and immunohistochemistry were performed to compare the ability of vessels formation functions between BM-MSCs and AD-MSCs or UC-MSCs. Results： The lengths and dots of vascular structures formed by EPCs on AD-MSCs layer are greater than those by EPCs on BM-MSCs layer and UC-MSCs layer in angiogenic assay in vitro. The stability of the capillary-like structures formed by EPCs with AD-MSCs on Matrigel was more stable than that by the BM-MSCs, UC-MSCs or EPCs. AD-MSCs and EPCs could form abundant functional vessels with blood perfusion in Matrigelin vivo; UC-MSCs and EPCs could form a few functional vessels with blood perfusion in Matrigelin vivo; BM-MSCs and EPCs could form broken vessels with hemocytes leakage in Matrigel in vivo. Conclusion： AD-MSCs have the stronger ability to promote the angiogenesis and stabilize the vessels compared with BM-MSCs or UC-MSCs ex vivo and in vivo.

关 键 词：人骨髓来源间充质干细胞 人脂肪来源间充质干细胞 人脐带来源间充质干细胞 内皮祖细胞 血管生成 血管稳定 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]