云南榅桲的薄层培养再生体系建立和多倍体诱导  

Establishment of Regeneration System from Transverse Cell Thin Layers and Polyploid Induction in Yunnan Quince(Cydonia oblonga Mill)

作　　者：房晨[1] 刘凤霞 王志刚[1] 杨成泉[1] 徐凌飞[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2017年第12期1860-1867,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家梨产业技术体系建设专项(CARS-29-40);西北农林科技大学渭南试验示范站建设专项~~

摘　　要：旨在建立云南榅桲的薄层培养再生体系,并以此为基础进行云南榅桲的多倍体诱导。以云南榅桲茎段薄层为外植体,系统研究各种因素对茎段薄层再生不定芽的影响,以及不同质量浓度秋水仙素对云南榅桲茎段薄层不定芽再生率以及多倍体植株诱导的影响。结果表明,云南榅桲薄层再生不定芽的适宜培养基组合为MS+TDZ 1.5mg·L-1+IBA 0.4mg·L-1+蔗糖30mg·L-1+琼脂7g·L-1,暗培养7d,再生频率最高为35.41%,平均再生芽数2.15。薄层再生苗在生根培养基1/2 MS+NAA 0.3 mg·L-1+蔗糖20g·L-1+琼脂7g·L-1上已经生根。利用薄层再生结合秋水仙素处理诱导出云南榅桲多倍体,经流式细胞仪鉴定,确定获得的多倍体为四倍体植株。To establish the regeneration system from transverse cell thin layers of Yunnan quince and induct polyploid Yunnan quince,the factors that influence adventitious buds regeneration from transverse cell thin layer were examined systematically.In the induction of tetraploid,proliferating shoots of Yunnan quince were treated with different concentrations of colchicine for 24 h,then tTCLs sections were excised from the nodal segments and were transferred to shoot regeneration medium.Optimum response to direct adventitious shoot bud induction from tTCLs was observed on MS medium containing 1.5 mg·L^（-1） TDZ,0.4 mg·L^（-1） IBA,30 g·L^（-1） sucrose,7.0 g·L^（-1） agar,and extra dark period of 7 days at the beginning of culture.The highest regeneration frequency of Yunnan quince was35.41%,and the mean bud number per tTCLs was 2.15.The regenerated shoots were rooted on 1/2 MS＋0.3 mg·L^（-1） NAA＋20 g·L^（-1） sucrose＋7 g·L^（-1） agar.The ploidy level of the colchicine treated individuals was analyzed by flow cytometry.The results show that two of the shoots regenerated from tTCLs with excised from shoots of Yunnan quince treated with colchicie were tetraploid.

关 键 词：云南榅桲 薄层培养 秋水仙素 多倍体诱导 

分 类 号：S661.2[农业科学—果树学]