大黄素对实验性自身免疫性甲状腺炎小鼠的免疫保护作用研究  

作　　者：夏亚男 李宁[1] 叶志鹏[1] 黄捷[1] 徐涛[1] 吴可人[1] 

机构地区：[1]浙江中医药大学附属第一医院,310006

出　　处：《浙江临床医学》2018年第3期413-415,共3页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药科技计划资助（2014ZA037）

摘　　要：目的探讨大黄素对实验性自身免疫性甲状腺炎小鼠的免疫保护作用和机制。方法通过过量碘剂诱导NOD小鼠建立EAT动物模型，造模4周后大黄素组小鼠进行大黄素灌胃处理。造模8周后检测小鼠血浆TgAb水平、甲状腺淋巴浸润程度及外周血T细胞亚群频率以观察大黄素对EAT小鼠的免疫保护作用。结果（1）甲状腺病理学观察，大黄素组较模型组甲状腺淋巴细胞浸润程度减轻，且两组淋巴细胞浸润程度均高于对照组，各组比较差异有统计学意义（P〈0．01）。（2）造模小鼠血浆TgAb水平均较对照组升高，且大黄素组升高幅度明显低于模型组，各组比较差异有统计学意义（P〈0．01）。（3）大黄素组外周血单核细胞中CD3＋CD4＋、CD3＋CD8＋T细胞频率和CD3＋CD4＋FIN-γ＋、CD3＋CD4＋IL-4＋T细胞频率均明显低于模型组，且均高于对照组，各组间比较差异有统计学意义（P〈0.01）。结论过量碘剂诱导NODd、鼠自身免疫性甲状腺炎造模是可行的；大黄素对造模小鼠甲状腺组织的自身免疫性炎症反应有一定免疫抑制作用，大黄素通过抑制CD4＋、CD8＋T淋巴细胞增殖分化及其IFN-γ和IL-4细胞因子分泌，从而发挥免疫性保护作用。Objective To investigate the immuno-protecrive effect of emodin on thyroid in experimental autoimmune thyroiditis （ EAT ） mice and its mechanism. Methods Four weeks after modeling, emodin group were treated by gavage with emodin. While eight weeks after modeling, The TgAb level in plasma, the degree of lymphocyte infiltration in thyroid and T cell subset frequency in peripheral blood were detected to evaluate the immuno-protecfive effect of emodin on EAT mice. Results （ 1 ） The histopathological study revealed that the degree of infiltration of thyroid lymphocyte was decreased in the emodin group compared with the model group, both were higher than that in the normal group, and there were significant differences among the each group （P〈0.01） . （2） Levels of plasma TgAb were increased in each group after modeling, and the increasing amplitudes in emodin treated groups were significandy less than that in model group （ P〈0.01 ） . （ 3 ） The cell frequencies of CD3＋CD4＋, CD3＋CD8＋T cells, CD3＋CD4＋IL-4＋ and CD3＋CD4＋IFN- γ＋ T cells in peripheral blood monocyte were significant decreased in emodin group compared with the model group, both were higher than the control group, and there were significant differences among each group （P〈0.01） . Conclusion The EAT model is visible which though an excessive iodine-induced method in NOD mice, and the emodin shows a certain inhibitory effect on autoimmune response in EAT mice. This effect can be performed by inhibiting the differentiation of lymphocyte into CD3＋CD4＋, CD3＋CD8＋T cells and inhibiting the secretion oflFN- γ and IL-4 in CD3＋CD4＋ T cells, thereby, immunological protection is performed.

关 键 词：NOD小鼠 实验性自身免疫性甲状腺炎 大黄素 T细胞亚群 TH1细胞 TH2细胞 

分 类 号：R581.4[医药卫生—内分泌]