人参提取液体外抑制IHNV作用效果研究  

Inhibition Effect Ginseng Extract on IHNV in vitro

作　　者：茆安婷 尹玉伟 代静 郭曦尧 程义 李东[1] 李月红[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《中国兽药杂志》2018年第2期31-35,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金(30972191)

摘　　要：探究人参提取液体外抑制CCB细胞感染IHNV的能力,构建体外CCB细胞模型,运用RTCA技术和MTT法测得不同浓度人参提取液对IHNV抑制作用曲线,并从阻断病毒复制和杀灭病毒两方面讨论人参提取液作用效果。结果表明,4%人参提取液对IHNV具有明显抑制作用,能够在16 h内保护细胞不受IHNV病毒的侵染,其中2 h时对病毒灭活抑制率最高达到51.5%,1~12 h内对病毒复制有持续抑制作用。研究可为IHVN防治药物的研发提供新的思路及参考。To delve the ability of Panax ginseng extractliquid inhibition IHNV infected CCB cells, CCB cell model was constructed in vitro, using RTCA technology and MTI＂ method the inhibitory action curve of ginseng extract on IHNV was measured, and discussing the effect of ginseng extracliquid from blocking virus replication and killing two aspects of the virus. The results showed that 4% ginseng extract liquidhad obvious inhibitory effect on IHNV, it can protect cells from infection by IHNV virus for a certain period of time, When 2 h, the inhibition rate of virus inactivation was highest, reaching 51.5%, IHVN prevention and treatment of drug research 1- 12 h has a continuous inhibitory effect on viral replication. and development to provide new ideas and reference

关 键 词：人参提取液 IHNV 抑制 

分 类 号：S853.74[农业科学—临床兽医学]