痛风康对痛风性关节炎大鼠TLR4/NF-κB通路的影响  

Effect of Tongfengkang on TLR4/NF-kappa B pathway in rats with acute gouty arthritis

作　　者：尤元梅 袁松 万毅[3] 

机构地区：[1]皖北卫生职业学院,安徽宿州234000 [2]宿州市中医院 [3]安徽中医药大学

出　　处：《包头医学院学报》2018年第1期95-98,共4页Journal of Baotou Medical College

摘　　要：目的:观察中药组方痛风康对痛风性关节炎大鼠的治疗作用,分析痛风康治疗痛风性关节炎的可能机制,并与治疗效果确切的西药吲哚美辛进行比较。方法:选择健康的SD大鼠48只,随机分为4组,分别为中药组、西药组、对照组、模型组,每组12只。中药组采用痛风康配置的溶液灌胃,西药组采用吲哚美辛溶液灌胃,对照组、模型组采用生理盐水灌胃。灌胃3 d后,除对照组外,其余各组大鼠均采用尿酸钠溶液注射大鼠右踝关节腔造模,建立急性痛风性关节炎大鼠模型。灌胃5 d后测量大鼠踝关节肿胀度,免疫组化法检测大鼠关节滑膜组织核转录因子κB(NF-κB)p65的表达,实时荧光定量PCR方法检测Toll样受体4(TLR4)mRNA、骨髓样分化因子88(MyD88)mRNA、肿瘤坏死因子受体相关因子6(TRAF6)mRNA的相对表达量,ELISA方法检测血清中白介素-8(IL-8)的含量。结果:与对照组相比,治疗前其余各组大鼠踝关节呈急性炎性红肿,关节肿胀度明显,治疗后,中药组及西药组大鼠踝关节肿胀度均减轻,关节滑膜组织TLR4 mRNA、MyD88 mRNA、TRAF6 mRNA相对表达量下降,NF-κB p65表达下降,血清中IL-8含量下降(均为P<0.05)。结论:痛风康治疗痛风性关节炎,其机制可能与对TLR4/NF-κB通路的干预有关。Objective：To observe the treatment effect of Tongfengkang on gouty arthritis of rats,to explore the possible mechanism in the treatment of gouty arthritis and to compare it with indomethacin with definite treatment efficiency.Methods：A total of 48 healthy SD rats were selected and randomly divided into Chinese medicine group,western medicine group,normal group and model group,12 rats in each group.The Chinese medicine group was treated with Tongfengkang solution,the western medicine group was treated with indomethacin solution,and the normal group and model group were treated with normal saline.3 days later,all rats except for those in the normal group were injected with sodium urate solution into the right ankle joint cavity to establish a model of gouty arthritis in rats.The swelling rate of ankle joint was measured after 5 days of treatment.The expression of NF-kappa Bp65 in synovial tissue of rats was detected by immunohistochemistry.The relative expressions of TLR4 mRNA,MyD88 mRNA and TRAF6 mRNA were detected by real-time quantitative PCR.ELISA method was used to detect the content of IL-8 in serum.Results：Compared with the normal group,the ankle joint of rats in model group showed acute inflammatory swelling,with significant joint swelling.After treatment,the swelling degree of ankle joint was alleviated in Chinese medicine group and Western medicine group.The relative expression of TLR4 mRNA,MyD88 mRNA and TRAF6 mRNA in synovial tissue decreased.The expression of NF-kappa Bp65 decreased,and the content of IL-8 in serum decreased（P〈0.05）.Conclusion：The mechanism of Tongfengkang in the treatment of acute gouty arthritis may be related to the intervention of the TLR4/NF-kappa B pathway.

关 键 词：痛风康 痛风性关节炎 实验 

分 类 号：R285.5[医药卫生—中药学]