西南地区部队官兵55型人腺病毒中和抗体的研究  

Study of neutralizing antibody against human adenovirus type 55 in soldiers of Southwest China

作　　者：刘媛[1] 王文博[2] 邹自英[1] 范泉水[2] 冯子良[2] 熊杰[1] 

机构地区：[1]成都军区总医院检验科,成都610083 [2]成都军区疾病预防控制中心,成都610021

出　　处：《第二军医大学学报》2018年第2期212-215,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81301445);全军后勤"十二五"重大专项(AWS11L009);全军医学科技青年培育项目(14QNP057);上海市医学生物防护重点实验室开放课题(SKLM1401)~~

摘　　要：目的探究西南地区部队官兵55型人腺病毒(HAd V55)抗体流行情况,并检测HAd V55抗体的中和活性,为部队预防和控制HAd V55疫情提供参考依据。方法收集西南地区部队官兵血清样本325份,用ELISA法检测血清中HAd V55抗体,通过体外中和实验检测抗体阳性血清中和HAd V55感染的活性。结果西南地区部队官兵体内HAd V55抗体的阳性率较低,325份血清中抗体阳性仅有19份,阳性率为5.85%。19份抗体阳性的血清中,14份在人喉癌上皮细胞系Hep-2具有中和活性,中和效价最高为1∶64。结论西南地区官兵体内HAd V55抗体水平较低,抵抗HAd V55感染的能力较弱。因此一旦出现个别感染者,部队应加强对HAd V55感染的预防和控制,以防止感染的扩散。Objective To investigate the prevalence and neutralizing activity of anti-human adenovirus type 55（antiHAd V55） among the soldiers in Southwest China, so as to provide reference for the prevention and control of HAd V55. Methods The sera of 325 soldiers in Southwest China were collected, and the anti-HAd V55 in serum was detected by ELISA. The neutralization activity of antibody positive serum was detected by neutralization test in vitro. Results The positive rate of anti-HAd V55 was low（5.85%, 19/325） among the soldiers in Southwest China. Of the 19 cases of positive serum, 14 cases were able to neutralize HAd V55 infection in human laryngeal epithelial carcinoma cell line（Hep-2 cells） with the highest titer being 1∶64. Conclusion The positive rate of anti-HAd V55 is low among the soldiers in Southwest China, which suggests the soldiers in Southwest China have a low resistance to HAd V55 infection. Once the individual infection is found, the troops should strengthen the prevention and control of HAd V55 infection to prevent the spreading of infection.

关 键 词：腺病毒 官兵 中国西南地区 血清 中和抗体 

分 类 号：R373.1[医药卫生—病原生物学]