高效液相色谱法测定热炎宁颗粒中野黄芩苷的含量  

Determination of scutellaria baicalensis georgi in Reynning granules by High Performance Liquid Chromatography

作　　者：刘恺[1] 刘海涛 梁俊[1] 曹银[1] 徐亚运[1] 戴彪[1] 聂丽娟 夏清荣[1] 

机构地区：[1]合肥市第四人民医院药剂科,230022 [2]合肥远志医药科技开发有限公司,合肥市230011

出　　处：《临床合理用药杂志》2018年第5期18-19,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的建立高效液相色谱法(HPLC)测定热炎宁颗粒中野黄芩苷含量的测定方法。方法色谱柱:Ultimate XB-C18柱(4.6×250 mm,5μm);以乙腈-0.02 mol/L磷酸二氢钾溶液(用三乙胺调节p H值至5.0)(14∶86)为流动相;检测波长:335 nm;柱温:30℃;流速:1.0 ml/min;进样量:20μl。结果野黄芩苷在7.57~30.28 mg/L的浓度范围内线性良好,平均加样回收率为100.11%,相对标准偏差为0.3%。结论该测定方法灵敏度高、准确、可靠、重现性良好,可作为热炎宁颗粒测定野黄芩苷的质量控制方法。Objective To establish an HPLC method for the determination of the content of scutellarin in Reyanning granules. Methods Ultimate XB-C18 column （250 × 4.6 mm,5 μm） was used. Acetonitrile and O. 02 mol/L monopotassium phosphate（ adjust the pH to 5.0 with triethylamine） at a ration of 14：86 was taken as the mobile phase. The sample was determined by HPLC at the wavelength of 335nm and the temperature of column was 30℃. The flow rate was 1.0 ml/min. Sample size was 20μl each time. Results Scutellarin had a good linear relationship within the range of 7.57 - 30.28 mg/L. The average recovery rates were 100.11% ,and relative standard deviation was 0.3%. Conclusion The method has the characteristics of high sensitivity, accuracy, reliability, and good reproducibility, which can be used as a quantitative analysis method for scutellarin in Reyanning granules.

关 键 词：热炎宁颗粒 野黄芩苷 高效液相色谱法 含量测定 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]