振荡与静置条件下细菌诱导形成的碳酸钙矿物类型与形态的对比  

Species and Morphologies of Calcium Carbonates Induced by Bacteria under Shake and Static Culture

作　　者：郭文文 李福春[2] 张宠宏[2] 

机构地区：[1]枣庄学院旅游与资源环境学院,枣庄277160 [2]南京农业大学资源与环境科学学院,南京210095

出　　处：《高校地质学报》2018年第1期33-40,共8页Geological Journal of China Universities

基　　金：国家自然科学基金项目(41673083;41172308);中国科学院黄土与第四纪地质国家重点实验室开放课题(SKLLQG1309);山东省自然科学基金项目(ZR2016DB34);枣庄学院博士科研启动基金项目(2013BS03)联合资助

摘　　要：为了探究培养方式(振荡和静置)对细菌诱导形成的碳酸盐矿物种类和形态的影响,本文在B4培养基中分别对蜡状芽孢杆菌(GW-1菌株)、赖氨酸芽孢杆菌(GW-2菌株)和微杆菌(GW-3菌株)三种细菌进行了为期40天的振荡培养实验及相应的无菌对照实验,重点利用X-射线衍射仪和扫描电子显微镜分别对矿物成分和形态进行了测定和观察,并将观测结果与静置条件下的结果进行了对比。结果表明:(1)三种细菌均具有促进碳酸盐矿物沉淀的功能,但能力有所不同,从大到小的顺序为GW-2>GW-1>GW-3;(2)在振荡和静置两种培养方式下,GW-1和GW-2菌株诱导形成的矿物均为方解石,而GW-3菌株诱导形成的矿物均为球霰石。这说明细菌种类是影响矿物相的重要因素,培养方式对矿物相无明显的影响;(3)对于GW-1和GW-2菌株,其在振荡培养条件下诱导形成的矿物形态呈现多样化,而在静置培养条件下形成的矿物形态则比较单一。对于GW-3菌株,其诱导形成的矿物均为球形,与培养方式无关。To better explore the effect of culture method(static and shake cultures) on species and morphologies of carbonate mineralsinduced by bacteria, a batch of oscillatory experiments were carried out for a duration of 40 days in B4 medium under the mediation ofthree kinds of bacteria-Bacillus cereus(strain GW-1), Lysinibacillus sp.( strain GW-2) and Microbacterium sp.(strain GW-3). At thesame time, aseptic experiment without the inoculation was done as the control. The mineral species of carbonate were determined byX-ray diffraction(XRD), and morphologies of precipitated carbonates were observed using scanning electron microscopy(SEM). Theresults obtained under oscillatory incubation condition were compared with those obtained under static condition. The main resultswere as follows:(1) All three bacterial strains could induce precipitate of calcium carbonate, but they had different abilities. Theirmineralization abilities were in the order of GW-2>GW-1>GW-3;(2) At the two culture methods, strains GW-1 and GW-2 induced the formation of calcite, while strain GW-3 mediated the formation of vaterite. The results suggested that bacterial species were the important factors affecting mineral species. Culture methods almost had no effect on mineral species;(3) The morphologiesof minerals induced by strains GW-1 and GW-2 were diversified with shake culture. However, single morphology existed in thesamples with static culture. The morphology of all minerals formed by strain GW-3 was spherical shape and was not related to theculture methods used.

关 键 词：方解石 球霰石 形态 细菌 振荡培养 静置培养 

分 类 号：P611[天文地球—矿床学]