过敏性鼻炎合并哮喘患者血液嗜酸性粒细胞群中P物质及其受体表达上调  

Upregulated expression of substance P and its receptor in blood eosinophils of patients with allergic rhinitis complicated with asthma

作　　者：臧艳艳 柴文戍 王君灵 张慧云 杨蕊铭 何韶衡 

机构地区：[1]锦州医科大学附属第一医院变态反应与临床免疫研究中心,辽宁锦州121001 [2]锦州医科大学附属第一医院呼吸内科,辽宁锦州121001

出　　处：《西安交通大学学报（医学版）》2018年第2期250-255,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金面上项目(No.81471592;81472016)~~

摘　　要：目的检测不同过敏原刺激下过敏性鼻炎合并哮喘(allergic rhinitis complicated with asthma,AR+AS)患者嗜酸性粒细胞群中P物质(substance P,SP)和NK1受体(neurokinin 1receptor,NK1R)的表达水平,并阐述其在疾病发生过程中的作用。方法募集14例健康人及19例急性期入院的AR+AS患者,所有志愿者进行皮肤点刺试验并收集外周血,用蒿草花粉、尘螨和梧桐花粉3种过敏原粗提液刺激后流式细胞术检测外周血嗜酸性粒细胞群中SP和NK1R的表达水平,并用SPSS软件进行数据分析。结果 19例AR+AS患者外周血嗜酸性粒细胞中,SP^+细胞的比例与正常人相比升高了1.5倍(Z=-2.041,P=0.041),NK1R^+细胞的比例和平均荧光强度分别上调了26.4%(Z=-3.207,P=0.001)和85.9%(Z=-4.774,P<0.001)。此外,质量浓度为0.1μg/mL的蒿草花粉粗提液刺激患者外周血后,SP^+细胞的比例上调约68.1%(Z=-2.637,P=0.008)。而健康人嗜酸性粒细胞群中SP和NK1R经不同种类和浓度过敏原刺激后其表达水平无统计学差异。结论嗜酸性粒细胞源SP和NK1R可能在AR+AS的发生过程中起重要作用,而SP和NK1R可能是治疗AR+AS的新靶点。Objective To detect the expression of substance P(SP)and NK1 receptor(NK1 R)in eosinophils of patients with allergic rhinitis complicated with asthma(AR+AS)and elucidate their roles in the pathogenesis.Methods Levels of SP and NK1 R in eosinophils were detected by flow cytometry after stimulation with crude extracts of Artemisia pollen,dust mite and Platanus pollen,respectively.Results The proportion of SP^+cells in patients with AR+AS was 1.5 folds higher than that of healthy controls(HCs)(Z=-2.041,P=0.041).The ratio of NK1 R^+cells and the mean fluorescent intensity were increased by 26.4%(Z=-3.207,P=0.001)and85.9%(Z=-4.774,P<0.001),respectively.In addition,0.1μg/mL of Artemisia pollen extract induced an increase of SP^+eosinophils in AR+AS patients by approximately 68.1%(Z=-2.637,P=0.008).However,no significant difference was detected in the expressions of SP and NK1 R in blood eosinophils of HCs when stimulated with allergens.Conclusion Eosinophil-derived SP and NK1 R may play an important role in the development of AR+AS.SP and NK1 R may be the potential targets for AR+AS treatment.

关 键 词：过敏性鼻炎合并哮喘 嗜酸性粒细胞 P物质 NK1受体 过敏原 

分 类 号：R392[医药卫生—免疫学]