泽泻汤加味方对高脂血症大鼠胃黏膜AQP3蛋白表达的影响  

Effect of Supplemented Formula of Alisma Decoction on Aquaporin-3 Expression in Gastric Mucosa of Hyperlipidemia Rats

作　　者：段思明 张兴芳 张一昕[1] 韩雪[1] 王亚芬[1] 刘晨旭 张纳博 王彦蕊 

机构地区：[1]河北中医学院,石家庄050200

出　　处：《河北中医药学报》2018年第1期1-4,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省中医药管理局科学技术研究计划资助项目:No.2016008;河北省高等学校科学技术研究项目:No.QN2015251;国家级大学生创新创业训练计划项目:No.201514432001

摘　　要：目的:研究泽泻汤加味方对高脂血症大鼠胃黏膜水通道蛋白3(AQP3)蛋白表达的影响,探讨其防治高脂血症的作用机制。方法:将60只健康SD大鼠随机分为空白组、模型组、辛伐他汀对照组(简称对照组)和泽泻汤加味方高、中、低剂量组,造模的同时给予相应药物治疗。第5周末,观察大鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)的含量及肝组织形态学变化,并运用免疫组化及Western Blot法检测大鼠胃黏膜中AQP3蛋白的表达变化。结果:模型组大鼠血清TG、TC、LDL-C含量升高、胃黏膜AQP3表达增强(P<0.01),HDL-C含量降低(P<0.01或P<0.05),各用药组大鼠TG、TC、LDL-C含量降低、胃黏膜AQP3的表达减弱,HDL-C含量升高(P<0.01或P<0.05)。结论:泽泻汤加味方具有调节血脂作用,通过下调胃黏膜AQP3的表达,调节水液代谢,祛除痰浊病理因素,可能是其防治高脂血症的作用机制之一。Objective： to study the effect of supplemented formula of Alisma decoction on Aquaporin-3（ AQP3） expression in gastric mucosa of hyperlipidemia rats,and to explore the mechanism of prevention and treatment of hyperlipidemia. Methods： 60 healthy SD rats were randomly divided into blank group,model group,Simvastatin control group（ short for control group）,and high dose,middle and low dose group of Alisma decoction. At the end of the fifth week,triglyceride（ TG）,total cholesterol（ TC）,high density lipoprotein（ HDL-C）,low density lipoprotein（ LDL-C） levels and liver histological changes in rat serum were observed and the expression of AQP3 protein was detected in rat gastric mucosa by immunohistochemistry and Western Blot method. Results： TG,TC,LDL-C levels of model group increased and AQP3 expression of gastric mucosa was enhanced（ P〈0. 01）,the content of HDL-C decreased（ P〈0. 01 or P〈0. 05）; of the medication groups,TG,TC,LDL-C levels decreased,AQP3 expression decreased,and HDL-C increased（ P〈0. 01 or P〈0. 05）. Conclusion： Supplemented formula of Alisma decoction has the effect of regulating blood lipid. It may be one of the mechanisms of preventing and treating hyperlipidemia by reducing the expression of AQP3 in gastric mucosa,regulating the metabolism of water and eliminating the pathological factors of phlegm turbidity.

关 键 词：泽泻汤加味方 高脂血症 痰浊 血瘀 水通道蛋白3 辛伐他汀 消痰祛浊 健运脾胃 

分 类 号：R285.5[医药卫生—中药学]