肺炎合并脓毒症患儿外周血调节性T细胞含量与炎症应激反应、免疫应答的相关性研究  

The correlation of peripheral blood regulatory T cell content with inflammatory stress response and immune response in children with both pneumonia and sepsis

作　　者：何源[1] 易海英[1] 杨树杰[1] 黄杰[1] 魏贤[1] 

机构地区：[1]武汉科技大学附属孝感医院新生儿科,湖北孝感432000

出　　处：《海南医学院学报》2018年第4期530-533,共4页Journal of Hainan Medical University

基　　金：湖北省医学科技计划项目(WJ2015Z124)~~

摘　　要：目的:探讨肺炎合并脓毒症患儿外周血调节性T细胞含量与炎症应激反应、免疫应答的相关性。方法:选择2014年9月~2017年4月间在本院接受治疗的肺炎合并脓毒症患儿90例作为脓毒症组,同期在本院进行疫苗接种的健康小儿100例作为正常对照组。对比两组小儿外周血调节性T细胞(Treg)表达量,血清中炎症介质、氧化应激指标、免疫球蛋白含量的差异。采用Pearson检验评估肺炎合并脓毒症患儿Treg表达量与病情的相关关系。结果:脓毒症组外周血中Treg的表达量明显高于正常对照组;血清中炎症介质降钙素原(PCT)、超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)含量高于正常对照组(P<0.05);血清中氧化应激指标一氧化氮(NO)、丙二醛(MDA)含量高于正常对照组,超氧化物歧化酶(SOD)含量低于正常对照组(P<0.05);免疫球蛋白IgA、IgM、IgG的含量低于正常对照组(P<0.05)。Pearson检验发现,肺炎合并脓毒症患儿外周血中Treg表达量与机体炎症应激反应、免疫应答功能均直接相关。结论:肺炎合并脓毒症患儿外周血Treg表达量显著增高,可直接影响机体炎症应激反应程度及体液免疫功能。Objective:To investigate the correlation of peripheral blood regulatory T cell content with inflammatory stress response and immune response in children with both pneumonia and sepsis.Methods:90 children with both pneumonia and sepsis who were treated in our hospital between September 2014 and April 2017 were selected as sepsis group and 100 healthy children who received vaccination in our hospital during the same period were selected as normal control group.The differences in peripheral blood regulatory T cell(Treg)expression as well as serum inflammatory mediator,oxidative stress index and immunoglobulin contents were compared between the two groups of children.Pearson test was used to evaluate the correlation between Treg expression and disease in patients with both pneumonia and sepsis.Results:Peripheral blood Treg expression of sepsis group was significantly higher than that of control group;serum inflammatory mediators PCT,hs-CRP,TNF-αand IL-6 contents were higher than those of control group;serum oxidative stress indexes NO and MDA contents were higher than those of control group whereas SOD content was lower than that of control group;immunoglobulin IgA,IgM and IgG contents were lower than those of normal control group.Pearson test showed that peripheral blood Treg expression of children with both pneumonia and sepsis was directly correlated with the inflammatory stress response and immune response.Conclusion:Peripheral blood Treg expression significantly increases in children with both pneumonia and sepsis,which could directly affect the inflammatory stress response extent and humoral immune function.

关 键 词：脓毒症 肺炎 调节性T细胞 炎症反应 氧化应激 免疫球蛋白 

分 类 号：R459.7[医药卫生—急诊医学]