球状C1q受体在自然流产中的表达及生物学意义  

Expression of globular C1q receptor in spontaneous abortion,and its biological significance

作　　者：闫林萍 高玲娟[2] 钟天鹰[3] 

机构地区：[1]南京医科大学第四临床医学院,江苏南京210004 [2]南京医科大学附属妇产医院(南京市妇幼保健院)医学研究中心,210004 [3]南京医科大学附属妇产医院(南京市妇幼保健院)检验科,210004

出　　处：《蚌埠医学院学报》2018年第1期4-7,共4页Journal of Bengbu Medical College

基　　金：江苏省南京市医学科技发展计划项目(YKK14124)

摘　　要：目的:探索球状C1q受体(gC1qR)在自然流产中的表达及其生物学意义。方法:收集自然流产和人工流产蜕膜组织各30例,以滋养层细胞系HTR-8/SVneo为研究对象,免疫组织化学SP法检测gC1qR蛋白在蜕膜组织中的表达及定位情况;免疫印迹技术及实时荧光定量聚合酶链反应分别检测gC1qR蛋白及mRNA的相对表达水平;流式细胞技术对滋养层细胞凋亡情况进行评估,透射电镜对滋养层细胞凋亡形态进行观察。结果:gC1qR蛋白及mRNA在自然流产蜕膜组织中的表达明显高于人工流产的蜕膜组织(P<0.01);过表达gC1qR基因可明显增加滋养层细胞的凋亡率(P<0.01),且电镜下呈现典型的凋亡形态学改变。结论:gC1qR基因与自然流产的发生紧密相关,有望成为诊断、治疗及预防自然流产的潜在靶点。Objective: To explore the expression of globular C1 q receptor( gC1qR) and its biological significance. Methods: Thirty decidual tissue specimen of spontaneous abortion and 30 samples of induced abortion were collected,and extravillous cytotrophoblasts( EVCT)-derived transformed cell lines HTR-8/Svneo was investigated. The protein expression and location of gC1qR in decidual tissue were detected using immunohistochemical SP method,the expression levels of gC1qR protein and mRNA were detected using immunofluorescent staining and qRT-PCR,the apoptosis of trophoblast cells was evaluated using flow cytometry and electronic microscope. Results: The expression levels of gC1qR protein and mRNA in decidual tissue in spontaneous abortion patients were significantly higher than those in induced abortion patients( P < 0. 01). The overexpression of gC1qR could significantly increase the apoptosis rate of trophoblast cells( P < 0. 01),and the typical apoptosis morphological change was observed under electronic microscope. Conclusions: The gC1qR gene is closely related to spontaneous abortion,which is maybe to be an potential target point in the diagnosis,treatment and prevention of spontaneous abortion.

关 键 词：自然流产 球状C1q受体 滋养层细胞 凋亡 

分 类 号：R714.21[医药卫生—妇产科学]