栀子大黄汤有效部位群分离纯化研究  

Isolation and Purification of the Effective Fractions of Zhi-Zi-Da-Huang Decoction

作　　者：李腾 马鑫 朱德康 徐峥 闻俊 周婷婷 

机构地区：[1]第二军医大学药学院,上海市药物(中药)代谢产物研究重点实验室,上海200433 [2]中国人民解放军92154部队医院,山东烟台264680

出　　处：《时珍国医国药》2018年第1期81-83,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81573584);第二军医大学本科生创新孵化基地项目(FH2016071)

摘　　要：目的建立并优化D101型大孔吸附树脂分离纯化栀子大黄汤有效部位群条件。方法采用紫外-可见分光光度法测定总环烯醚萜苷、总异黄酮、总黄酮、总蒽醌的含量,以总环烯醚萜苷、总异黄酮、总黄酮、总蒽醌的含量作为考察指标,分别考察了上样量、上样浓度、洗脱剂浓度等因素对分离纯化工艺的影响。结果最佳工艺条件为上样量:树脂量(g∶ml)=1∶5;上样浓度按原药材计为0.5g/ml;吸附时间为3 h;每个浓度的洗脱剂用量为6个柱体积,流速控制2个柱体积/h。30%乙醇、60%乙醇、70%乙醇可以分别将总环烯醚萜苷、总黄酮、总异黄酮和总蒽醌洗脱完全。结论该分离纯化工艺合理、稳定,可用于工业生产。Objective To isolate and purify the effective fractions of Zhi - Zi - Da - Huang Decoction, D - 101 macroporous resin was used to separate the components with the optimal conditions. Methods UV - Vis spectrophotometry was used to measure the total content of iridoid glycoside, flavone, isoflavone and anthraquinone contained in these effective fractions. The content of total iridoid glycoside, isoflavones, flavonoids and anthraquinone were taken as the targets to investigated the influence of the sample weight, sample concentration and the concentration of eluent on the separation and purification process. Results The optimal ratio of loading amount of extractive to resin concentration was 1：5 （g：ml）. The optimum sample concentration was 0.5g/ml in terms of the raw materials. 30% ethanol, 60% ethanol and 70% ethanol could completely elute iridoid glycosides, flavonoids, isofla- vones and anthraquinone, respectively. Conclusion This optimized separation and purification technology was reasonable and sta- ble, and it could be extended to large -scale production applications.

关 键 词：栀子大黄汤 总环烯醚萜苷 总黄酮 总异黄酮 总蒽醌 

分 类 号：R284.1[医药卫生—中药学]